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Series GSE42054 Query DataSets for GSE42054
Status Public on Nov 03, 2014
Title Diverse phospho-signaling networks mediate RTK dependent growth and survival in childhood ALL [copy number]
Organism Homo sapiens
Experiment type Genome variation profiling by SNP array
Summary Deregulated RTK activity has been implicated as a causal leukemogenic factor in the context of molecular aberrations that perturb differentiation in the hematopoietic lineage such as in childhood ALL. A deeper understanding of RTK signaling processes on a system-wide scale will be key in defining critical components of signaling networks. To link RTK activity with in vivo output in primary ALL we took a functional approach, which combined SH2 domain binding, mass spectrometry, and transcriptome analyses. Structure and composition of evolving networks were highly diverse with few generic features determined by receptor and cell type. A combinatorial assembly of varying context-dependent and few generic signaling components at multiple levels likely generates output specificity. PAK2 was identified as a phosphoregulated FLT3 target, whose allosteric inhibition resulted in apoptosis of ALL cells. Our studies provide evidence that a functional approach to leukemia signaling may yield valuable information for a network-directed intervention.
 
Overall design Primary ALL samples were investigated on Affymetrix SNP 6.0 arrays for copy-number changes related to receptor tyrosine kinases (RTKs). Buffy-Coat samples of healthy persons were used as reference.
 
Contributor(s) Dierck K, Horstmann M
Citation(s) 30301811
Submission date Nov 06, 2012
Last update date Nov 27, 2018
Contact name Thomas Streichert
Organization name University Medical Center Hamburg-Eppendorf
Department Institute For Clinical Chemistry / Central Laboratories
Street address Martinistr. 52, Campus Forschung N27, 2. OG
City Hamburg
ZIP/Postal code D-20246
Country Germany
 
Platforms (1)
GPL6801 [GenomeWideSNP_6] Affymetrix Genome-Wide Human SNP 6.0 Array
Samples (12)
GSM1031463 ALL24429
GSM1031464 ALL22089
GSM1031465 ALL119
This SubSeries is part of SuperSeries:
GSE42040 Diverse phospho-signaling networks mediate RTK dependent growth and survival in childhood ALL
Relations
BioProject PRJNA179035

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42054_CBS_derived_segments.txt.gz 46.7 Kb (ftp)(http) TXT
GSE42054_Dierck.vs.Buffy.smoothed.signal.ratios.scaled.txt.gz 34.6 Mb (ftp)(http) TXT
GSE42054_RAW.tar 353.0 Mb (http)(custom) TAR (of CEL)
Processed data are available on Series record

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