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Series GSE41833 Query DataSets for GSE41833
Status Public on Feb 06, 2013
Title Expression profiles of primary bone marrow derived mouse macrophages – untreated and treated with LPS or LPS+PGE
Organism Mus musculus
Experiment type Expression profiling by array
Summary The polarization of macrophages into an anti-inflammatory or regulatory phenotype plays an important role in resolving inflammation. PGE2 regulates macrophage polarization via a PKA dependent pathway. PKA phosphorylates SIKs, inhibiting their ability to phosphorylate CRTC3 in cells. This in turn allows CRTC3 to translocate to the nucleus where it acts as a co-activator with the transcription factor CREB to induce IL-10 transcription. In line with this we find that either genetic or pharmacological inhibition of SIKs mimics the effect of PGE2 on IL-10 production.
We show here that PGE2, in combination with LPS, is able to promote a regulatory like phenotype in macrophages characterized by high expression of IL-10 as well as the regulatory markers SPHK1 and LIGHT.
Overall design Gene expression profiles of primary bone marrow derived mouse macrophages treated with LPS or LPS+PGE and untreated controls were generated using Affymetrix mouse gene 1.1 ST arrays. In total, 12 arrays were generated from the three groups (LPS, LPS+PGE and control) with 4-replicates in each group. One sample from LPS group was removed due to QC issues and the remaining 11 that were used in our analyses are submitted here.
Contributor(s) Arthur SJ, Mudaliar MA, Barton GJ, Elcombe S, MacKenzie K
Citation(s) 23241891
Submission date Oct 25, 2012
Last update date Apr 18, 2017
Contact name Manikhandan A V Mudaliar
Organization name University of Glasgow
Department College of Medical, Veterinary and Life Sciences
Lab Institute of Infection, Immunity and Inflammation
Street address Room B528, Sir Graeme Davies Building, 120 University Place
City Glasgow
State/province Scotland
ZIP/Postal code G12 8TA
Country United Kingdom
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (11)
GSM1025298 macrophages_untreated_control_rep1
GSM1025299 macrophages_untreated_control_rep2
GSM1025300 macrophages_untreated_control_rep3
BioProject PRJNA178264

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41833_RAW.tar 46.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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