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Series GSE41680 Query DataSets for GSE41680
Status Public on Oct 19, 2012
Organism Homo sapiens
Experiment type Expression profiling by array
Summary EGFR activation is important in human epithelial malignancies, including cutaneous squamous cell carcinoma, lung, colon, pancreatic and other cancers. Therapies targeting EGFR are currently used to treat such cancers, but one significant drawback to EGFR inhibitor therapies is the associated skin toxicity. This toxicity usually presents as papular or pustular folliculitis, dry skin with pruritus and hair and nails abnormalities. The side effects often limit the usefulness of EGFR inhibitors in cancer treatment. The transcriptional changes caused by EGFR inhibition in epidermal keratinocytes have not been extensively explored. To define the transcriptional changes caused by inhibition of EGFR in primary human epidermal keratinocytes, we treated these cells with Tyrphostin and compared treated and control cultures in parallel, using Affymetrix microarrays. Using metaanalysis approaches, we integrated the observed changes with a large set of already existing data on transcriptional profiling in epidermal keratinocytes. We found that EGFR inhibition suppresses expression of genes associated with keratinocyte proliferation, attachment and motility. Apoptosis is facilitated by both induction of proapoptotic and suppression of antiapoptotic genes. Surprisingly, EGFR inhibition induces expression of markers of epidermal differentiation.
Overall design Time course of human epidermal keratinocytes treated with Tyrphostin (AG1478) and untreated controls
Contributor(s) Blumenberg M, Gazel A
Citation Gazel A, Blumenberg M, Transcriptional effects of inhibiting epidermal growth factor receptor in keratinocytes, Dermatologica Sinica (2012),
Submission date Oct 18, 2012
Last update date Dec 06, 2018
Contact name Miroslav Blumenberg
Phone 212 263-5924
Organization name NYU School of Medicine
Department Dermatology
Lab Blumenberg
Street address 455 First Ave #874
City New York
State/province NY
ZIP/Postal code 10016
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (8)
GSM1022408 Control culture at 1 h
GSM1022409 Control culture at 4 h
GSM1022410 Control culture at 24 h
BioProject PRJNA177919

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41680_RAW.tar 34.2 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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