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Series GSE41677 Query DataSets for GSE41677
Status Public on Jan 17, 2013
Title Latent enhancers unveiled by stimulation expand and adapt the available cis-regulatory repertoire (FAIRE-seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary According to current models, transcription factors (TFs) activated by extracellular stimuli operate in the context of a pre-established enhancer repertoire induced and maintained by lineage-specific TFs. Here, we uncovered the existence of latent enhancers, defined as regions of the genome that in terminally differentiated cells are poorly accessible and lack the histone marks characteristic of enhancers, but readily acquire these features in response to extracellular cues. Stimulation of resting macrophages caused simultaneous binding of stimulus-activated TFs and lineage-determining TFs to these regions, enabling deposition of enhancer-specific features. Once unveiled, these enhancers did not return to a latent state even when stimulation ceased; instead, they persisted and mediated a faster and stronger response upon restimulation. We suggest that stimulus-specific expansion of the available cis-regulatory repertoire provides an epigenomic memory of the exposure to environmental agents.
Overall design Formaldehyde-Assisted Isolation Of Regulatory Elements (FAIRE) followed by multiparallel sequencing was performed in untreated murine bone marrow-derived macrophages.
Contributor(s) Ostuni R, Piccolo V, Barozzi I, Polletti S, Ghisletti S, Natoli G
Citation(s) 23332752
Submission date Oct 17, 2012
Last update date May 15, 2019
Contact name Iros Barozzi
Organization name Medical University Vienna
Street address Borschkegasse 8a
City Vienna
ZIP/Postal code 1090
Country Austria
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (1)
This SubSeries is part of SuperSeries:
GSE38379 Latent enhancers unveiled by stimulation expand and adapt the available cis-regulatory repertoire
BioProject PRJNA177885
SRA SRP016512

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Supplementary file Size Download File type/resource
GSE41677_RAW.tar 310.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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