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Series GSE41602 Query DataSets for GSE41602
Status Public on Aug 09, 2013
Title Wapl controls higher-order chromatin structure in interphase chromosomes (ChIP-Seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Mammalian genomes contain several billion base pairs of DNA which are packaged in chromatin fibers. At selected gene loci, cohesin complexes have been proposed to arrange chromatin fibers into higher-order structures, but it is poorly understood how cohesin performs this task, how important this function is for determining the structure of chromosomes, and how this process is regulated to allow changes in gene expression. Here we show that the cohesin release factor Wapl controls chromatin structure and gene regulation at numerous loci throughout the mouse genome. Conditional deletion of the Wapl gene leads to stable accumulation of cohesin on chromatin, chromatin compaction, altered gene expression, cell cycle delay, chromosome segregation defects and embryonic lethality. In Wapl deficient chromosomes, cohesin accumulates in an axial domain, similar to how condensins form a “scaffold” in mitotic chromosomes. We propose that Wapl controls chromatin structure and gene regulation by determining the residence time with which cohesin binds to DNA.
Overall design ChIP-Seq using two different antibodies (CTCF, Smc3); one (CTCF) and two (Smc3) replicates; two different genotypes (Wapl +/delta, Wapl -/delta). The control sample is a single-replicate INPUT for each genotype.
Contributor(s) Wuensche A, Schirghuber E, Wutz G, Ellenberg J, Peters J, Jaritz M, Huet S, Nishiyama T, Venugopal B, Tedeschi A
Citation(s) 23975099
Submission date Oct 15, 2012
Last update date May 15, 2019
Contact name Roman R Stocsits
Organization name IMP - Research Institute of Molecular Pathology
Lab Jan-Michael Peters' Lab
Street address Campus-Vienna-Biocenter 1
City Vienna
ZIP/Postal code 1030
Country Austria
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (8)
GSM1020006 Smc3 Wapl +/delta rep1
GSM1020007 Smc3 Wapl -/delta rep1
GSM1020008 Smc3 Wapl +/delta rep2
This SubSeries is part of SuperSeries:
GSE41603 Wapl controls higher-order chromatin structure in interphase chromosomes
BioProject PRJNA177691
SRA SRP016126

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41602_RAW.tar 799.7 Mb (http)(custom) TAR (of BED, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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