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Series GSE41473 Query DataSets for GSE41473
Status Public on Oct 11, 2012
Title Gene expression profiling of C/EBPβ- and STAT3-deficient human endometrial stromal cells (HESC)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Our previous studies have shown that C/EBPβ plays a critical role in human endometrial stromal decidualization. In order to identify the molecular pathways regulated by C/EBPβ during decidualization, we performed gene expression profiling using RNA isolated from normal and C/EBPβ-deficient human endometrial stromal cells. The microarray results revealed that several key regulators of stromal differentiation, such as BMP2, Wnt4, IL-11Rα and STAT3, operate downstream of C/EBPβ during decidualization. Further studies revealed that STAT3 is a direct target of C/EBPβ and plays an important role in cytokine signal during the decidualization process. Gene expression profiling, using STAT3-deficient HESCs, showed an extensive overlap of pathways downstream of STAT3 and C/EBPβ during stromal cell differentiation.
Overall design We employed a siRNA strategy to suppress C/EBPβ or STAT3 mRNA expression in HESCs and then performed microarray analysis to identify its downstream target genes. Further, using a similar strategy, we focused on STAT3, a C/EBPβ target gene, and identified the commone pathways downstream of both C/EBPβ and STAT3.
Contributor(s) Wang W, Bagchi MK, Bagchi IC
Citation(s) 23097472
Submission date Oct 10, 2012
Last update date Dec 06, 2018
Contact name Wei Wang
Organization name University of Illinois
Department comparative biosciences
Street address 2001 S Lincoln Ave.
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (8)
GSM1017920 HESCs, Control siRNA, rep1
GSM1017921 HESCs, C/EBP beta siRNA, rep1
GSM1017922 HESCs, Control siRNA, rep2
BioProject PRJNA177117

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Supplementary file Size Download File type/resource
GSE41473_RAW.tar 16.3 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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