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Series GSE41460 Query DataSets for GSE41460
Status Public on Aug 09, 2013
Title Wapl controls higher-order chromatin structure in interphase chromosomes (gene expression)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mammalian genomes contain several billion base pairs of DNA which are packaged in chromatin fibers. At selected gene loci, cohesin complexes have been proposed to arrange chromatin fibers into higher-order structures, but it is poorly understood how cohesin performs this task, how important this function is for determining the structure of chromosomes, and how this process is regulated to allow changes in gene expression. Here we show that the cohesin release factor Wapl controls chromatin structure and gene regulation at numerous loci throughout the mouse genome. Conditional deletion of the Wapl gene leads to stable accumulation of cohesin on chromatin, chromatin compaction, altered gene expression, cell cycle delay, chromosome segregation defects and embryonic lethality. In Wapl deficient chromosomes, cohesin accumulates in an axial domain, similar to how condensins form a “scaffold” in mitotic chromosomes. We propose that Wapl controls chromatin structure and gene regulation by determining the residence time with which cohesin binds to DNA.
Overall design 4 biological replicates for each genotype (Wapl +/F; Wapl -/F) treated with/without 4-OHT =16 samples
Contributor(s) Wuensche A, Schirghuber E, Wutz G, Ellenberg J, Peters J, Jaritz M, Huet S, Nishiyama T, Venugopal B, Tedeschi A
Citation(s) 23975099
Submission date Oct 10, 2012
Last update date Sep 20, 2013
Contact name Roman R Stocsits
Organization name IMP - Research Institute of Molecular Pathology
Lab Jan-Michael Peters' Lab
Street address Campus-Vienna-Biocenter 1
City Vienna
ZIP/Postal code 1030
Country Austria
Platforms (1)
GPL16159 IMP Genomics Mouse 58MM
Samples (16)
GSM1017652 MEF_Wapl(+/F)+4OHT_1
GSM1017653 MEF_Wapl(+/F)_1
GSM1017654 MEF_Wapl(+/F)+4OHT_2
This SubSeries is part of SuperSeries:
GSE41603 Wapl controls higher-order chromatin structure in interphase chromosomes
BioProject PRJNA177069

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41460_RAW.tar 31.7 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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