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Series GSE41449 Query DataSets for GSE41449
Status Public on Aug 31, 2014
Title Epigenetic regulation of gene expression is instructed by promoter sequence and refined by developmental progression [expression]
Organism Mus musculus
Experiment type Expression profiling by array
Summary A large body of literature suggests that local features of chromatin are crucial in determining functional properties of underlying DNA. The extent to which DNA sequence plays an active role in the establishment and maintenance of patterns of histone and DNA modification at promoters in cells and tissues in an adult animal remains poorly understood. Likewise, whether passage through development is required for refinement of transcriptional states and chromatin marks characteristic of fully differentiated adult cells remains unclear. Here we undertook analysis of gene expression along with the genomic distribution of DNA methylation and a set of histone marks in primary tissues derived from different primordial germ layers in adult mice. We find that promoter CpG content correlated strongly with specific repressive mechanisms. Likewise, RNA polymerase II occupancy and active histone marks were omnipresent on CpG rich promoters regardless of transcriptional output and of cell type. However, these same chromatin features cleanly demarcate transcriptional activity on promoters with low CpG content. While these observations imply an instructive role for DNA sequence in establishment and maintenance of epigenetic states, experimental evidence presented here also suggests an additional role for development in refining these features at individual promoters.
Overall design Gene expression in B cells and liver was measured using Agilent Whole Mouse Genome 4x44 multiplex format oligo arrays.
Contributor(s) Li R, Wade PA, Mav D, Shah RR
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Oct 10, 2012
Last update date Jan 12, 2017
Contact name Deepak Mav
Organization name Sciome LLC
Street address 2 Davis Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (5)
GSM1017580 B-cells, rep1
GSM1017581 B-cells, rep2
GSM1017582 Liver, rep1
This SubSeries is part of SuperSeries:
GSE41450 Epigenetic regulation of gene expression is instructed by promoter sequence and refined by developmental progression.
BioProject PRJNA177171

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41449_RAW.tar 45.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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