|
| Status |
Public on Oct 05, 2012 |
| Title |
Ago HITS-CLIP in KSHV-infected primary effusion lymphoma (PEL) cell lines BCBL-1 and BC-3 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We performed Ago HITS-CLIP to identify targets of viral and human miRNAs in latently KSHV-infected PEL cells
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| Overall design |
Ago HITS-CLIP was performed in two latently infected PEL cell lines, BCBL-1 and BC-3; Argonaute-immunoprecipitation of UV cross-linked Ago-miRNA-mRNA complexes, followed by RNA isolation, library construction, and high-throughput sequencing (Illumina GAxII); we performed 3 biological replicates for each cell line, two technical (sequencing) replicates of BCBL-1 biological replicate 1
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| |
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| Contributor(s) |
Haecker I, Gay LA, Yang Y, Hu J, Morse AM, McIntyre LM, Renne R |
| Citation(s) |
22927820 |
| |
| Submission date |
Oct 04, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Rolf Renne |
| Organization name |
University of Florida
|
| Street address |
2033 Mowry Road
|
| City |
Gainesville |
| ZIP/Postal code |
32608 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA176666 |
| SRA |
SRP016003 |
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