GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE4133 Query DataSets for GSE4133
Status Public on Aug 28, 2006
Title The Genome Wide Distribution of Acetylated Histone H4 Remodelled through Human Primary Myoblast Differentiation
Organism Homo sapiens
Experiment type Expression profiling by array
Genome binding/occupancy profiling by SNP array
SNP genotyping by SNP array
Summary The simultaneous genotyping of tens of thousands of SNP using SNP microarrays is a very important tool that is revolutionizing genetics and molecular biology. In this work, we present a new application of this technique by using it to assess chromatin immunoprecipitation (CHIP) as a means to assess the multiple genomic locations bound by a protein complex recognized by an antibody. We illustrate the use of this technique with an analysis of the change in histone H4 acetylation, a marker of open chromatin and transcriptionally active genomic regions, which occur during the differentiation of human myoblasts into myotubes. Our results are validated by the observation of a significant correlation between the histone modifications detected and the expression of the nearby genes, as measured by DNA microarrays.

This SuperSeries is composed of the SubSeries listed below.
Overall design This super series is composed of the following subset series:
GSE4131: Triplicate Affymetrix HGU133A/B expression chips were hybridized to RNA extracted from myoblasts and myotubes.
GSE4132: Triplicate Affymetrix 10K ax 13339 SNP chips were hybridized to DNA from myoblasts and myotubes.
Citation(s) 17868463
Submission date Jan 31, 2006
Last update date Aug 10, 2018
Organization Ottawa Hospital Research Institute
Phone (613) 737-8899 -73255
Department Cellular and Molecular Medicine
Lab Ottawa Bioinformatics Core Facility
Street address 501 Smyth Rd.
City Ottawa
State/province ON
ZIP/Postal code K1H 8L6
Country Canada
Platforms (3)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL97 [HG-U133B] Affymetrix Human Genome U133B Array
GPL3400 Affymetrix GeneChip Mapping Pre-Release 10K Array (ax13339)
Samples (21)
GSM93880 Myoblasts_rep1, chip HG-U133A
GSM93882 Myoblasts_rep1,chip HG-U133B
GSM93883 Myoblasts_rep2, chip HG-U133A
This SuperSeries is composed of the following SubSeries:
GSE4131 Determination of myotube and myoblast expression levels
GSE4132 Mapping of H4-acetylated chromatin modifications in differentiating human primary myoblasts
BioProject PRJNA95013

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4133_RAW.tar 66.5 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap