|Public on Aug 28, 2006
|The Genome Wide Distribution of Acetylated Histone H4 Remodelled through Human Primary Myoblast Differentiation
|Expression profiling by array
Genome binding/occupancy profiling by SNP array
SNP genotyping by SNP array
|The simultaneous genotyping of tens of thousands of SNP using SNP microarrays is a very important tool that is revolutionizing genetics and molecular biology. In this work, we present a new application of this technique by using it to assess chromatin immunoprecipitation (CHIP) as a means to assess the multiple genomic locations bound by a protein complex recognized by an antibody. We illustrate the use of this technique with an analysis of the change in histone H4 acetylation, a marker of open chromatin and transcriptionally active genomic regions, which occur during the differentiation of human myoblasts into myotubes. Our results are validated by the observation of a significant correlation between the histone modifications detected and the expression of the nearby genes, as measured by DNA microarrays.
This SuperSeries is composed of the SubSeries listed below.
|This super series is composed of the following subset series:
GSE4131: Triplicate Affymetrix HGU133A/B expression chips were hybridized to RNA extracted from myoblasts and myotubes.
GSE4132: Triplicate Affymetrix 10K ax 13339 SNP chips were hybridized to DNA from myoblasts and myotubes.
|Jan 31, 2006
|Last update date
|Aug 10, 2018
|Ottawa Hospital Research Institute
|(613) 737-8899 -73255
|Cellular and Molecular Medicine
|Ottawa Bioinformatics Core Facility
|501 Smyth Rd.
|[HG-U133A] Affymetrix Human Genome U133A Array
|[HG-U133B] Affymetrix Human Genome U133B Array
|Affymetrix GeneChip Mapping Pre-Release 10K Array (ax13339)
|This SuperSeries is composed of the following SubSeries:
|Determination of myotube and myoblast expression levels
|Mapping of H4-acetylated chromatin modifications in differentiating human primary myoblasts