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Series GSE41286 Query DataSets for GSE41286
Status Public on Jul 31, 2014
Title Targeting Unique Metabolic Properties of Breast Tumor Initiating Cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Bulk cancer cell populations are known to display distinct metabolic properties compared to their normal counterparts. However, relatively little is known about heterogeneity of metabolic properties within tumors. In this study we show that, analogous to some normal stem cells, breast tumor initiating cells (TICs, also called cancer stem cells) have distinct metabolic properties compared to non-tumorigenic cancer cells (NTCs). Transcriptome profiling using RNA-Seq revealed TICs under-express genes involved in mitochondrial oxidative phosphorylation and although TICs are relatively quiescent, they preferentially perform glycolysis over oxidative phosphorylation compared to NTCs. TICs contain fewer mitochondria and display lower expression and activity of pyruvate dehydrogenase (Pdh), a key regulator of oxidative phosphorylation. Metabolic reprogramming of TICs by pharmacologic activation of Pdh preferentially eliminates TICs in vitro and in vivo. Our findings reveal unique metabolic properties of TICs and indicate that metabolic reprogramming represents a promising strategy for targeting these cells.
Overall design Examination transcriptome profiles for breast tumor initiating cells (TICs) and non-tumorigenic cells (NTCs)
Contributor(s) Feng W, Gentles A, Nair RV, Diehn M
Citation(s) 24497069
Submission date Oct 02, 2012
Last update date May 15, 2019
Contact name Andrew Gentles
Organization name Stanford University
Street address 1201 Welch Road
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM1013599 TIC RNA-seq
GSM1013600 NTC RNA-seq
BioProject PRJNA176451
SRA SRP015968

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41286_RAW.tar 1.0 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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