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Status |
Public on May 19, 2013 |
Title |
Single-cell transcriptomics reveals widespread heterogeneity in gene regulation and RNA processing in stimulated immune cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Here, we used single-cell RNA-Seq to discover extensive cell-to-cell variability – in transcript expression levels, cell state, circuit usage, and alternative splicing – between stimulated immune cells
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Overall design |
RNA seq libraries from 18 single cells, 3 populations of 10,000 cells, and 2 zero cells (negative controls) with SMARTer. Three additional single cell libraries with molecular barcodes (MB) using a modified SMARTer protocol.
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Contributor(s) |
Shalek AK, Satija R, Park H, Regev A |
Citation(s) |
23685454 |
Submission date |
Oct 01, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Rahul Satija |
E-mail(s) |
rsatija@nygenome.org
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Phone |
6177022468
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Organization name |
New York Genome Center
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Lab |
Satija Lab
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Street address |
101 Avenue of the Americas
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City |
New York City |
State/province |
NY |
ZIP/Postal code |
10013 |
Country |
USA |
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Platforms (1) |
GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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Samples (24)
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Relations |
BioProject |
PRJNA176414 |
SRA |
SRP015959 |