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Series GSE41265 Query DataSets for GSE41265
Status Public on May 19, 2013
Title Single-cell transcriptomics reveals widespread heterogeneity in gene regulation and RNA processing in stimulated immune cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here, we used single-cell RNA-Seq to discover extensive cell-to-cell variability – in transcript expression levels, cell state, circuit usage, and alternative splicing – between stimulated immune cells
Overall design RNA seq libraries from 18 single cells, 3 populations of 10,000 cells, and 2 zero cells (negative controls) with SMARTer. Three additional single cell libraries with molecular barcodes (MB) using a modified SMARTer protocol.
Contributor(s) Shalek AK, Satija R, Park H, Regev A
Citation(s) 23685454
Submission date Oct 01, 2012
Last update date May 15, 2019
Contact name Rahul Satija
Phone 6177022468
Organization name New York Genome Center
Lab Satija Lab
Street address 101 Avenue of the Americas
City New York City
State/province NY
ZIP/Postal code 10013
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (24)
GSM1012777 Single cell S1
GSM1012778 Single cell S2
GSM1012779 Single cell S3
BioProject PRJNA176414
SRA SRP015959

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41265_allGenesTPM.txt.gz 1.0 Mb (ftp)(http) TXT
GSE41265_umbExp.txt.gz 253.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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