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Series GSE41252 Query DataSets for GSE41252
Status Public on Jan 01, 2013
Title Transcription factors interfering with dedifferentiation induce direct conversion
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Although transcription factor(TF)s regulate differentiation-related processes, including dedifferentiation and direct conversion, functional interactions between TFs regulating these processes are not well understood. Here we show that TFs preventing dedifferentiation are able to induce direct conversion. Using a neural lineage cell line and a large number of TFs expressed in it, we found a subset of TFs whose overexpression strongly interfered with dedifferentiation triggered by a procedure to induce induced pluripotent stem cells (iPSC), through a maintenance mechanism of the cell-type-specific transcriptional profile. Strikingly, the maintenance activity of the interfering TF set was strong enough to induce the cell line-specific transcriptional profile when overexpressed in a heterologous cell type. In addition, TFs that interfered with dedifferentiation in hepatic lineage cells involved known TFs with induction activity for hepatic lineage cells. Our results suggest that dedifferentiation suppresses a cell-type-specific transcriptional profile, which is primarily maintained by a small subset of TFs capable of inducing direct conversion. We anticipate that this functional correlation might be applicable in various cell types, which may include cancer cells, and might facilitate identification of TFs with induction activity to understand differentiation and tumorigenesis
Overall design Examination of binding of 3 transcription factors and histone modifications in Neural progenitor like cells.
Contributor(s) Masui S, Watanabe A
Citation(s) 23550161
Submission date Oct 01, 2012
Last update date May 15, 2019
Contact name Akira Watanabe
Organization name Kyoto University
Department Medical Innovation Center
Street address Shogoin-Kawahara-cho 53, Sakyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 606-8507
Country Japan
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (4)
GSM1012188 H32_N31_EGFP_OK_flag_2
GSM1012189 H36_NS-F-Pax6
GSM1012190 H38_NS-F-Etv6
BioProject PRJNA176339
SRA SRP015950

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41252_RAW.tar 2.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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