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Series GSE41090 Query DataSets for GSE41090
Status Public on Apr 09, 2014
Title Mouse CD4+/CD8+ thymocytes from miR-181a1/b1 KO vs WT
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary miR-181a1/b1, miR-181a2/b2, and miR-181c/d belong to a highly conserved family of microRNA clusters, yet their role in vivo is poorly understood. Here we show that the miR-181a1/b1 cluster is absolutely essential for NKT development and is a critical determinant of thymocyte proliferation, survival and T-cell receptor α locus rearrangement. Furthermore, while individual ablation of miR-181a2/b2 and miR-181c/d revealed no overt phenotypes, compound mutant mice lacking both miR-181a1/b1 and miR-181a2/b2 display decreased survival, reduced body weight, and abnormal B cell development. Mechanistically, we reveal that miR-181 regulates PTEN, a key tumor suppressor whose abundance determines key metabolic adaptations required to meet the biosynthetic demands of highly proliferative tissues. These results provide important insights into the physiological function of this family of microRNAs in vivo; moreover, it places miR-181 as a central regulator of the PI3K signaling pathway and cellular metabolism.
Overall design Three sorted populations of double positive (DP) thymocytes from each of two conditions (WT and miR181a1/b1 -/- mice)
Contributor(s) Henao-Mejia J, Williams A, Goff LA, Staron M, Licona-Limon P, Kaech SM, Nakayama M, Rinn JL, Flavel RA
Citation(s) 23623381
Submission date Sep 23, 2012
Last update date May 15, 2019
Contact name Loyal A Goff
Organization name Johns Hopkins University
Department Institute of Genetic Medicine
Lab Goff
Street address 733 N. Broadway Avenue
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM1008468 mir181a1/b1 Knockout DP thymocytes replicate 1
GSM1008469 mir181a1/b1 Knockout DP thymocytes replicate 2
GSM1008470 mir181a1/b1 Knockout DP thymocytes replicate 3
BioProject PRJNA175759
SRA SRP015851

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