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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 09, 2014 |
| Title |
Mouse CD4+/CD8+ thymocytes from miR-181a1/b1 KO vs WT |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
miR-181a1/b1, miR-181a2/b2, and miR-181c/d belong to a highly conserved family of microRNA clusters, yet their role in vivo is poorly understood. Here we show that the miR-181a1/b1 cluster is absolutely essential for NKT development and is a critical determinant of thymocyte proliferation, survival and T-cell receptor α locus rearrangement. Furthermore, while individual ablation of miR-181a2/b2 and miR-181c/d revealed no overt phenotypes, compound mutant mice lacking both miR-181a1/b1 and miR-181a2/b2 display decreased survival, reduced body weight, and abnormal B cell development. Mechanistically, we reveal that miR-181 regulates PTEN, a key tumor suppressor whose abundance determines key metabolic adaptations required to meet the biosynthetic demands of highly proliferative tissues. These results provide important insights into the physiological function of this family of microRNAs in vivo; moreover, it places miR-181 as a central regulator of the PI3K signaling pathway and cellular metabolism.
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| Overall design |
Three sorted populations of double positive (DP) thymocytes from each of two conditions (WT and miR181a1/b1 -/- mice)
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| Contributor(s) |
Henao-Mejia J, Williams A, Goff LA, Staron M, Licona-Limon P, Kaech SM, Nakayama M, Rinn JL, Flavel RA |
| Citation(s) |
23623381 |
| Submission date |
Sep 23, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Loyal A Goff |
| E-mail(s) |
loyalgoff@jhmi.edu
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| Organization name |
Johns Hopkins University
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| Department |
Institute of Genetic Medicine
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| Lab |
Goff
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| Street address |
733 N. Broadway Avenue
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (6)
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| GSM1008468 |
mir181a1/b1 Knockout DP thymocytes replicate 1 |
| GSM1008469 |
mir181a1/b1 Knockout DP thymocytes replicate 2 |
| GSM1008470 |
mir181a1/b1 Knockout DP thymocytes replicate 3 |
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| Relations |
| BioProject |
PRJNA175759 |
| SRA |
SRP015851 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE41090_WT_vs_KO_full_genes.tab.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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