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Series GSE40909 Query DataSets for GSE40909
Status Public on Sep 18, 2012
Title Conversion of human fibroblasts into vascular cells (methylation)
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary We report a novel technique to reprogram human fibroblasts into endothelial and smooth muscle cells using partial iPSC reprogramming and chemically defined media. Using appropriate media conditions for differentiation of human pluripotent cells to CD34+ vascular progenitor cells, we show that temporary expression of pluripotent transcription factors and treatment with chemically-defined media, will induce differentiation of human fibroblasts to CD34+ vascular progenitor cells. Sorted CD34+ cells can then be directed to differentiate into vascular endothelial cells expressing a variety of smooth muscle markers.
Overall design We have assessed the global DNA methylation (Illumina Infinium HD 450K DNA methylationBeadChips) and transcriptional (Illumina HT12v4 Gene Expression Bead Array) profiles of transdifferentiated endothelial cells and smooth muscle, human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) differentiated CD34+ angioblasts, hESCs, hiPSC, primary smooth muscle and primary human umbilical vein endothelial cells using microarrays.
Contributor(s) Kurian L, Sancho-Martinez I, Nivet E, Moon K, Pendaries C, Volle-Challier C, Bono F, Herbert J, Xia Y, Li M, Pulecio J, Monserrat N, Ruiz S, Aguirre A, Dubova I, Boscolo FS, Thiagarajan R, Loring JF, Laurent LC, Belmonte JC
Citation(s) 23202434
Submission date Sep 14, 2012
Last update date Mar 22, 2019
Contact name Rathi D Thiagarajan
Organization name UCSD
Department Reproductive Medicine
Lab Louise Laurent
Street address 2880 Torrey Pines Scenic Drive
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (25)
GSM1004625 Primary_Fibroblast_p4_631.1768.1.2
GSM1004626 Primary_Fibroblast_p5_608.1708.2.1
GSM1004627 Primary_Fibroblast_p14_621.1709.2.1
This SubSeries is part of SuperSeries:
GSE40927 Conversion of human fibroblasts into vascular cells
BioProject PRJNA175382

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40909_RAW.tar 183.1 Mb (http)(custom) TAR
GSE40909_methylation_unprocessed.txt.gz 62.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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