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Series GSE40813 Query DataSets for GSE40813
Status Public on Mar 01, 2013
Title macroH2A histone variants act as a barrier upon reprogramming towards pluripotency
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Here we report that the histone variant macroH2A acts as a barrier to induced pluripotency. Using fibroblasts isolated from macroH2A double knockout mice, we observed enhanced reprogramming efficiency compared to fibroblasts from wild type animals. We further show that macroH2A isoforms act synergistically in this process. Genomic analysis in wild type fibroblasts reveals that macroH2A1 and H3K27me3 domains co-localize and occupy pluripotency genes. While the absence of macroH2A does not affect H3K27me3 in fibroblasts, macroH2A1 is highly enriched at a set of Utx target genes that are reactivated early during iPS reprogramming.
Overall design Mononucleosomes from Dermal Fibroblasts (from wt and macroH2A1 and macroH2A2 double knockout mice) were isolated and ChIP'd with mH2A1, H3K27me3 and H3K27ac antibodies. DNA from Input and ChIP samples was purified and sequenced on Illumina's Hiseq.
Contributor(s) Bernstein E, Gaspar-Maia A, Hasson D
Citation(s) 23463008
Submission date Sep 12, 2012
Last update date May 15, 2019
Contact name Emily Bernstein
Phone (212) 824-9335
Organization name Mount Sinai School of Medicine
Department Oncological Sciences
Lab Bernstein Lab
Street address 1470 Madison Avenue, 6th floor Rm 302
City New York
State/province NY
ZIP/Postal code 10029
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (8)
GSM1002446 Input control
GSM1002447 wt_macroH2A1
GSM1002448 wt_H3K27me3
BioProject PRJNA175035
SRA SRP015724

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Supplementary file Size Download File type/resource
GSE40813_RAW.tar 6.8 Gb (http)(custom) TAR (of BED, TXT, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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