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Series GSE40712 Query DataSets for GSE40712
Status Public on Sep 09, 2012
Title Expression data from CD34+ hematopoietic cells transduced with control or anti-HCLS1 shRNA
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Knockdown of HCLS1 mRNA in CD34+ hematopoietic cells resulted in a severe diminished in vitro myeloid differentiation which was in line with downregulation of a set of genes, e.g., of Wnt or PI3K/Akt signaling cascades. We performed microarrays to evaluate specific genes and signaling systems regulated by HCLS1 in hematopoietic cells.
Overall design CD34+ hematopoietic cells were isolated from the bone marrow of healthy individuals, transduced with lenitvirus-based RFP-expressed shRNA constructs targeting HCLS1 mRNA (two different shRNA constructs were used simultaneously) or scrambled control shRNA. After 4 days of the transductions, RFP+ cells were sorted, and total RNA of sorted cells was isolated and used for Affymetrix Exome arrays.
Contributor(s) Skokowa J, Welte K
Citation(s) 23001182
Submission date Sep 09, 2012
Last update date Jan 25, 2019
Contact name Siarhei Kandabarau
Organization name Hannover Medical School
Street address Carl-Neuberg-Str 1
City Hannover
ZIP/Postal code 30625
Country Germany
Platforms (1)
GPL10739 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version]
Samples (2)
GSM999604 Ctrl shRNA
GSM999605 HCLS1 shRNA
BioProject PRJNA174739

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Supplementary file Size Download File type/resource
GSE40712_RAW.tar 8.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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