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Series GSE40369 Query DataSets for GSE40369
Status Public on Dec 20, 2012
Title The Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiation
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The Mediator complex functions as a control center orchestrating diverse signalings, gene activities, and biological processes. However, how Mediator subunits determine distinct cell fates remains to be fully elucidated. Here, we show that Mediator MED23 controls the cell fate preference that directs differentiation into smooth muscle cells (SMCs) or adipocytes. Med23-deficiency facilitates SMC differentiation but represses adipocyte differentiation from the multipotent mesenchymal stem cells. Gene profiling revealed that the presence or absence of Med23 oppositely regulates two sets of genes: the RhoA/MAL-targeted cytoskeleton/SMC genes and the Ras/ELK1 targeted growth/adipocyte genes. Mechanistically, MED23 favors ELK1-SRF binding to SMC gene promoters for repression, whereas the lack of MED23 favors MAL-SRF binding to SMC gene promoters for activation. Remarkably, the effect of MED23 on SMC differentiation can be recapitulated in zebrafish embryogenesis. Collectively, our data demonstrate the dual, opposing roles for MED23 in regulating the cytoskeleton/SMC and growth/adipocyte gene programs, suggesting its “Ying-Yang” function in directing adipogenesis vs. SMC differentiation.
Overall design Examination of SRF enrichment in sictrl and si23 10T1/2 cells
Contributor(s) Yin J, Yao X, Wang G
Citation(s) 22972934
Submission date Aug 24, 2012
Last update date May 15, 2019
Contact name Jingwen Yin
Phone 86-21-54921085
Organization name SIBCB, CAS
Street address yueyang road
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (2)
GSM992343 jwyin_srf_sictrl_ChIPSeq
GSM992344 jwyin_srf_si23_ChIPSeq
BioProject PRJNA173867
SRA SRP015135

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Supplementary file Size Download File type/resource
GSE40369_RAW.tar 780.5 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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