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Series GSE40350 Query DataSets for GSE40350
Status Public on Nov 01, 2012
Title Mechanistic and structural insight into the functional dichotomy between interleukin-2 and interleukin-15
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Interleukin-15 (IL-15) and IL-2 possess distinct immunological functions despite both signaling through IL-2Rβ and the common cytokine receptor γ-chain, γc, We find that in the IL-15/IL-15Rα/IL-2Rβ/γc quaternary complex structure, IL-15 heterodimerizes IL-2Rβ and γc identically to the IL-2/IL-2Rα/IL-2Rβ/γc complex, despite differing receptor-binding chemistries. IL-15Rα dramatically increases the affinity of IL-15 for IL-2Rβ, and this allostery is required for IL-15 trans-signaling versus IL-2 cis-signaling. Consistent with the identical IL-2Rβ/γc dimer geometry, IL-2 and IL-15 exhibited similar signaling properties in lymphocytes, with any differences resulting from disparate receptor affinities. Thus, IL-15 and IL-2 induce similar signals, and the cytokine-specificity of IL-2Rα versus IL-15Rα determines cellular responsiveness. These results provide important new insights for specific development of IL-15- versus IL-2-based immunotherapeutics.
Overall design RNA-Seq is conducted in mouse CD8+ T cells, not treated or treated with IL2 or IL15 for indicated concentrations (1nM or 500nM) and times (4hr or 24hr).
Contributor(s) Ring AM, Lin J, Feng D, Mitra S, Rickert M, Bowman GR, Pande V, Li P, Moraga I, Spolski R, Özkan E, Leonard WJ, Garcia K
Citation(s) 23104097
Submission date Aug 23, 2012
Last update date May 15, 2019
Contact name Peng Li
Organization name NIH
Department NHLBI
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (9)
GSM991797 Cont RNASeq
GSM991798 IL2 1nM 4h RNASeq
GSM991799 IL2 1nM 24h RNASeq
BioProject PRJNA173649
SRA SRP015113

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Supplementary file Size Download File type/resource
GSE40350_RAW.tar 3.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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