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| Status |
Public on Sep 21, 2012 |
| Title |
Human monocyte-derived DCs: effect of IL-4 superkines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression induced by IL-4 and IL-4 superkines on CD14+ monocytes cultured in the presence of GM-CSF. Super-4 and KFR signal preferentially through the type I and type II IL-4 receptor, respectively.
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| Overall design |
Five biological replicates (n=5 healthy donors), four conditions: GM-CSF alone or in combination with IL-4, Super-4 or KFR. Two colors: Cy3 for GM-CSF alone, Cy5 for any combination. Cells were cultured for 6 hours with 50 ng/ml of GM-CSF and 20 ng/ml of IL-4, Super-4 or KFR.
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| Contributor(s) |
Creusot RJ, Moraga I, Fathman CG, Garcia KC |
| Citation(s) |
23103943 |
| Submission date |
Aug 17, 2012 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Remi J Creusot |
| E-mail(s) |
rcreusot@stanford.edu
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| URL |
http://fathmanlab.stanford.edu/people/index.html
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| Organization name |
Stanford University
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| Department |
Medicine
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| Lab |
Fathman
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| Street address |
269 Campus Dr, CCSR 2240
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (16)
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| GSM988218 |
Donor 1, GM (Cy3) vs. GM+IL-4 (Cy5) |
| GSM988219 |
Donor 1, GM (Cy3) vs. GM+IL-4 (Cy5), replicate |
| GSM988220 |
Donor 1, GM (Cy3) vs. GM+Super-4 (Cy5) |
| GSM988221 |
Donor 1, GM (Cy3) vs. GM+KFR (Cy5) |
| GSM988222 |
Donor 2, GM (Cy3) vs. GM+IL-4 (Cy5) |
| GSM988223 |
Donor 2, GM (Cy3) vs. GM+Super-4 (Cy5) |
| GSM988224 |
Donor 2, GM (Cy3) vs. GM+KFR (Cy5) |
| GSM988225 |
Donor 3, GM (Cy3) vs. GM+IL-4 (Cy5) |
| GSM988226 |
Donor 3, GM (Cy3) vs. GM+Super-4 (Cy5) |
| GSM988227 |
Donor 3, GM (Cy3) vs. GM+KFR (Cy5) |
| GSM988228 |
Donor 4, GM (Cy3) vs. GM+IL-4 (Cy5) |
| GSM988229 |
Donor 4, GM (Cy3) vs. GM+Super-4 (Cy5) |
| GSM988230 |
Donor 4, GM (Cy3) vs. GM+KFR (Cy5) |
| GSM988231 |
Donor 5, GM (Cy3) vs. GM+IL-4 (Cy5) |
| GSM988232 |
Donor 5, GM (Cy3) vs. GM+Super-4 (Cy5) |
| GSM988233 |
Donor 5, GM (Cy3) vs. GM+KFR (Cy5) |
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| Relations |
| BioProject |
PRJNA173078 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40200_RAW.tar |
338.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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