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Series GSE40132 Query DataSets for GSE40132
Status Public on Feb 15, 2013
Title ALKBH5 is a New Mammalian RNA Demethylase
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary N6-methyladenosine (m6A) is the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Here we report ALKBH5 as a new mammalian demethylase that oxidatively removes the m6A modification in mRNA in vitro and inside cells. This demethylation activity of ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly of mRNA processing factors in nuclear speckles. Alkbh5-deficient male mice are characterized by impaired fertility resulting from apoptosis that affects meiotic metaphase-stage spermatocytes. In accordance with this defect, we have identified in mouse testes 1552 differentially expressed genes which cover broad functional categories and include spermatogenesis-related mRNAs involved in the p53 functional interaction network. We show that Alkbh5-deficiency impacts the expression levels of some of these mRNAs, supporting the observed phenotype. The discovery of this new RNA demethylase strongly suggests that the reversible m6A modification plays fundamental and broad functions in mammalian cells.
Overall design RNA-seq in two cell types
Contributor(s) Zheng G, Lu Z
Citation(s) 23177736
Submission date Aug 15, 2012
Last update date May 15, 2019
Contact name zhike lu
Organization name University of Chicago
Department department of chemistry
Street address 5801 South Ellis Avenue
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (4)
GSM986109 HeLa siControl RNASeq
GSM986110 HeLa siALKB5 RNASeq
GSM986111 mouse wt RNASeq
SRA SRP014857
BioProject PRJNA173338

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40132_RAW.tar 5.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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