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| Status |
Public on Dec 22, 2015 |
| Title |
Expression data from Amacr knock-out mouse intestine |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Bile acids play multiple roles in vertebrate metabolism by facilitating lipid absorption in the intestine and acting as a signaling molecule in lipid and carbohydrate metabolism. Bile acids are also the main route to excrete excess cholesterol out of the body. Alpha-methyl-Coa racemase (Amacr) is one of the enzymes needed to produce bile acids from cholesterol. The mouse model lacking Amacr can produce only minor (less than 10%) amounts of bile acids, but still they are symptomless in normal laboratory conditions.
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| Overall design |
Cholesterol absorption occurs in the jejunum part of the intestine. In this experiment, the intestines from Amacr-/- and wild-type mice were divided into four equal segments and the endothelial layer was collected by scraping. The RNA from these samples was isolated and the gene expression levels in each segment were compared. 3 biological replicates per genotype/segment combination.
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| Contributor(s) |
Autio KJ, Kvist AP |
| Citation(s) |
26248199 |
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| Submission date |
Aug 14, 2012 |
| Last update date |
Dec 22, 2015 |
| Contact name |
Kaija J Autio |
| E-mail(s) |
kaija.autio@oulu.fi
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| Phone |
+358 294 48 1142
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| Organization name |
University of Oulu
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| Department |
Faculty of Biochemistry and Molecular Medicine
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| Street address |
P.O.Box 5400
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| City |
University of Oulu |
| ZIP/Postal code |
90014 |
| Country |
Finland |
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| Platforms (1) |
| GPL14661 |
[Mouse430_2_Mm_EntrezG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 13] |
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| Samples (24)
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| GSM986018 |
wild-type mouse intestine, segment 1, biological rep1 |
| GSM986019 |
wild-type mouse intestine, segment 1, biological rep2 |
| GSM986020 |
wild-type mouse intestine, segment 1, biological rep3 |
| GSM986021 |
Amacr-/- mouse intestine, segment 1, biological rep1 |
| GSM986022 |
Amacr-/- mouse intestine, segment 1, biological rep2 |
| GSM986023 |
Amacr-/- mouse intestine, segment 1, biological rep3 |
| GSM986024 |
wild-type mouse intestine, segment 2, biological rep1 |
| GSM986025 |
wild-type mouse intestine, segment 2, biological rep2 |
| GSM986026 |
wild-type mouse intestine, segment 2, biological rep3 |
| GSM986027 |
Amacr-/- mouse intestine, segment 2, biological rep1 |
| GSM986028 |
Amacr-/- mouse intestine, segment 2, biological rep2 |
| GSM986029 |
Amacr-/- mouse intestine, segment 2, biological rep3 |
| GSM986030 |
wild-type mouse intestine, segment 3, biological rep1 |
| GSM986031 |
wild-type mouse intestine, segment 3, biological rep2 |
| GSM986032 |
wild-type mouse intestine, segment 3, biological rep3 |
| GSM986033 |
Amacr-/- mouse intestine, segment 3, biological rep1 |
| GSM986034 |
Amacr-/- mouse intestine, segment 3, biological rep2 |
| GSM986035 |
Amacr-/- mouse intestine, segment 3, biological rep3 |
| GSM986036 |
wild-type mouse intestine, segment 4, biological rep1 |
| GSM986037 |
wild-type mouse intestine, segment 4, biological rep2 |
| GSM986038 |
wild-type mouse intestine, segment 4, biological rep3 |
| GSM986039 |
Amacr-/- mouse intestine, segment 4, biological rep1 |
| GSM986040 |
Amacr-/- mouse intestine, segment 4, biological rep2 |
| GSM986041 |
Amacr-/- mouse intestine, segment 4, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE40125 |
Expression data from Amacr knock-out mouse liver and intestine |
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| Relations |
| BioProject |
PRJNA172866 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40124_RAW.tar |
85.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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