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Series GSE40104 Query DataSets for GSE40104
Status Public on Apr 30, 2014
Title High-throughput sequencing of Brf1 (Zfp36l1) and Brf2 (Zfp36l2) targets
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We conducted RNA/RNA binding protein immunoprecipitation (RIP) followed by high throughput sequencing to identify mRNA targets of Brf1 (Zfp36l1) and Brf2 (Zfp36l2) in mouse embryonic stem cells. We collected over 190 million sequencing reads and identified many highly enriched protein coding mRNAs. Gene ontology analysis of enriched genes reveals that transcription factors and intercellular signaling proteins account for a large fraction of the targets, many of which are important for pluripotency and differentiation.
Overall design Identification of Brf1 and Brf2 mRNA targets
Contributor(s) Tan F, Elowitz M
Citation(s) 24733888
Submission date Aug 14, 2012
Last update date May 15, 2019
Contact name Frederick E Tan
Phone 626-395-5969
Organization name California Institute of Technology
Department Biology and Applied Physics
Lab Michael Elowitz
Street address 1200 E. California Blvd, M/C 114-96
City Pasadena
State/province CA
ZIP/Postal code 91125
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM984964 Total RNA 1
GSM984965 Total RNA 2
GSM984966 Sample immunoprecipitate 1
SRA SRP014839
BioProject PRJNA172921

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40104_FPKM.txt.gz 640.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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