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Series GSE40027 Query DataSets for GSE40027
Status Public on Jan 01, 2022
Title GM-CSF cultured and LPS stimulated WT and miR-155 KO DC
Organism Mus musculus
Experiment type Expression profiling by array
Summary Analyze the transcriptome pattern of miR-155 deficient BMDC in both steady-state and inflammatory conditions, in comparison to WT counterparts.
Overall design Total bone marrow was cultured in 40 ng/mL GM-CSF enriched RPMI-media for 7 days. A fraction of the floating cells were stimulated with 100 ng/mL LPS for 16 h, after with both unstimulated and stimulate DC were sorted by FACS for DC markers and activation markers (CD86). Unactivated DC from unstimulated samples (CD86-) were isolated together with activated (adherent) DC from LPS stimulated samples (CD86+), and total RNA was isolated.
Contributor(s) Hedlund S, Yuzawa Y, Fukao T
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Submission date Aug 10, 2012
Last update date Jan 01, 2022
Contact name Yoko Fukuda Yuzawa
Organization name Max-Planck Institute of Immunobiology
Street address Stübeweg 51
City Freiburg
ZIP/Postal code 79108
Country Germany
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (8)
GSM983895 WT_s_rep2
GSM983896 WT_u_rep1
GSM983897 WT_u_rep2
BioProject PRJNA172416

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40027_RAW.tar 73.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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