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Series GSE39982 Query DataSets for GSE39982
Status Public on Aug 10, 2012
Title Rapid Genome-Scale Mapping of Chromatin Accessibility in Tissue
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Here we introduce a novel technique that specifically identifies Tissue Accessible Chromatin (TACh). The TACh method uses pulverized frozen tissue as starting material and employs one of the two robust endonucleases, Benzonase or Cyansase, which are fully active under a range of stringent conditions such as high levels of detergent and DTT. As a proof of principle we applied TACh to frozen mouse liver tissue. Combined with massive parallel sequencing TACh identifies accessible regions that are associated with euchromatic features and accessibility at transcriptional start sites correlates positively with levels of gene transcription.
Overall design Idetification of accessible chromatin in forzen adult mouse liver tissue
Contributor(s) Grontved L, Hager GL, Levens D
Citation(s) 22734930
Submission date Aug 08, 2012
Last update date May 15, 2019
Contact name Songjoon Baek
Organization name NCI / NIH
Department CCR
Street address 41 Library Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (7)
GSM982941 Dnase I
GSM982942 Benzonase 0.25U
GSM982943 Benzonase 1U
BioProject PRJNA172402
SRA SRP014753

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Supplementary file Size Download File type/resource
GSE39982_Pooled3Benz_hs.bed.gz 494.6 Kb (ftp)(http) BED
GSE39982_Pooled3Cyan_hs.bed.gz 468.4 Kb (ftp)(http) BED
GSE39982_PooledLiverDHS_sat.bed.gz 583.5 Kb (ftp)(http) BED
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