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Status |
Public on Jul 05, 2013 |
Title |
Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses [Expt: Geni_Bafi] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Within the overall project, we performed a set of microarray and chromatin-immunoprecipitation (ChIP)-chip experiments using siRNA against the (pro)renin receptor ((P)RR), stable overexpression of PLZF, the PLZF translocation inhibitor genistein and the specific V-ATPase inhibitor bafilomycin to dissect transcriptional pathways downstream of the (P)RR.
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Overall design |
In this dataset, we include the expression data obtained from KELLY cells incubated with genistein or bafilomycin A1. Five intervention samples and three control samples were analyzed. We generated the following pairwise comparisons using Chipinspector (Genomatix Software GmbH) and a FDR of 0.5% (bafilomycin), 5% (genistein): genistein- versus DMSO- and bafilomycin- versus DMSO-treated cells. ChipInspector carries out significance analysis on the single probe level. Normalized probe set level data not provided for individual Sample records. Processed data is available on Series record.
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Contributor(s) |
Funke-Kaiser H, Zaade D |
Citation(s) |
23469216 |
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Submission date |
Aug 08, 2012 |
Last update date |
Jan 25, 2019 |
Contact name |
Daniela Zaade |
E-mail(s) |
Daniela.Zaade@charite.de, Daniela.Zaade@gmx.de
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Phone |
0049(0)30450525304
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Organization name |
Charité
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Department |
Center for Cardiovascular Research
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Lab |
AG Heiko Funke-Kaiser
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Street address |
Hessische Straße 3-4
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City |
Berlin |
ZIP/Postal code |
10115 |
Country |
Germany |
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Platforms (1) |
GPL10739 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version] |
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Samples (8)
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GSM982320 |
KELLY, genistein incubation, rep 1 |
GSM982321 |
KELLY, genistein incubation, rep 3 |
GSM982322 |
KELLY, bafilomycin incubation, rep 1 |
GSM982323 |
KELLY, bafilomycin incubation, rep 2 |
GSM982324 |
KELLY, bafilomycin incubation, rep 3 |
GSM982325 |
KELLY, DMSO control, rep 1 |
GSM982326 |
KELLY, DMSO control, rep 2 |
GSM982327 |
KELLY, DMSO control, rep 3 |
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This SubSeries is part of SuperSeries: |
GSE39965 |
Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses |
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Relations |
BioProject |
PRJNA172170 |
Supplementary file |
Size |
Download |
File type/resource |
GSE39962_FK_71-73_vs_77-78-79.txt.gz |
6.4 Kb |
(ftp)(http) |
TXT |
GSE39962_FK_74-75-76_vs_77-78-79.txt.gz |
18.4 Kb |
(ftp)(http) |
TXT |
GSE39962_RAW.tar |
34.3 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data are available on Series record |
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