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Series GSE39949 Query DataSets for GSE39949
Status Public on Feb 08, 2013
Title Gene expression profiling of human endometrial stromal cells transduced with BMP2- and WNT4-adenovirus.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Our previous studies have shown that bone morphogenetic protein 2 (BMP2), a morphogen belonging to the TGFβ superfamily, is markedly induced in human primary endometrial stromal cells (HESC) as they undergo differentiation in response to steroid hormones and cAMP. WNT4 is a downstream target of BMP2 regulation in these cells.
To identify the common downstream targets of BMP2 and WNT4 in human endometrial stromal cells, we performed gene expression profling of human ensometrial stromal cell transduced with BMP2 or WNT4 adenovirus. Gene expression profiling revealed that FOXO1, a forkhead family transcription factor and a known regulator of HESC differentiation, is a common downstream mediator of both BMP2 and WNT4 signaling. These studies uncovered a linear pathway involving BMP2, WNT4, and FOXO1 that operates in human endometrium to critically control decidualization.
Overall design Human endometrial stromal cells were transduced with recombinant adenovirus expressing BMP2, WNT4, or a negative control GFP at MOI 50:1 in 2 ml of culture medium. After transduction for 24 h, the viral particles were removed and the cells were treated with E+P for 3 days to induce decidualization (n=3 for each treatment), pooled total RNA from these cells was then hybridized to high density affymetrix microarrays according to the Affymetrix protocol (Human Genome HG-U133 A2.0 Array) .
Contributor(s) Li Q, Bagchi IC
Citation(s) 23142810
Submission date Aug 07, 2012
Last update date Dec 06, 2018
Contact name Quanxi Li
Phone 217-333-1731
Organization name Universit of Illinois at Urbana-Champaign
Department Comparative Bioscience
Lab Dr. Bagchi
Street address 2001 S lincoln Avenue
City Urbana
State/province IL
ZIP/Postal code 61802
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (3)
GSM982146 GFP-overexpressing human endometrial stromal cells
GSM982147 BMP2-overexpressing human endometrial stromal cells
GSM982148 WNT4-overexpressing human endometrial stromal cells
BioProject PRJNA172146

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Supplementary file Size Download File type/resource
GSE39949_RAW.tar 6.4 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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