|
| Status |
Public on Feb 10, 2013 |
| Title |
A comprehensive view of the transcriptome during development of the mouse cerebral cortex |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The complexity of the mature adult brain is a result of both developmental processes and experience-dependent circuit formation. One way to look at the process of brain development is to examine gene expression changes, and previous studies have used microarrays to address this in a global manner. However, the transcriptome is more complex than gene expression levels alone, as both alternative splicing and RNA editing occur to generate a more diverse set of mature transcripts. The aim of the current study was to develop a high-resolution transcriptome dataset of mouse cortical development using RNA sequencing (RNA-Seq), thus assaying exon usage and RNA editing as well as overcoming some of the inherent limitations of microarrays. We found a large number of differentially expressed genes, but also altered splicing and RNA editing between embryonic and adult cerebral cortex. Each dataset was validated both technically and biologically, and in each case tested we found our RNA-Seq observations to have high predictive validity. We propose this dataset, and the accompanying analysis, to be a helpful resource in the understanding of changes in gene expression during development.
|
| |
|
| Overall design |
Three young adult cerebral cortices four embryonic cerebral cortices
|
| |
|
| Contributor(s) |
Dillman AA, Hauser DN, Gibbs JR, Nalls MA, McCoy M, Kaganovich A, Rudenko IN, Galter D, Cookson MR |
| Citation(s) |
23416452 |
| Submission date |
Aug 03, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
J Raphael Gibbs |
| E-mail(s) |
gibbsr@mail.nih.gov
|
| Organization name |
National Institutue on Aging, NIH
|
| Department |
Computational Biology Core
|
| Lab |
Laboratory of Neurogenetics
|
| Street address |
35 Convent Drive, Bldg 35/1A1015, MSC3707
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA171892 |
| SRA |
SRP014662 |
Less...
More...