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Series GSE39866 Query DataSets for GSE39866
Status Public on Feb 10, 2013
Title A comprehensive view of the transcriptome during development of the mouse cerebral cortex
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The complexity of the mature adult brain is a result of both developmental processes and experience-dependent circuit formation. One way to look at the process of brain development is to examine gene expression changes, and previous studies have used microarrays to address this in a global manner. However, the transcriptome is more complex than gene expression levels alone, as both alternative splicing and RNA editing occur to generate a more diverse set of mature transcripts. The aim of the current study was to develop a high-resolution transcriptome dataset of mouse cortical development using RNA sequencing (RNA-Seq), thus assaying exon usage and RNA editing as well as overcoming some of the inherent limitations of microarrays. We found a large number of differentially expressed genes, but also altered splicing and RNA editing between embryonic and adult cerebral cortex. Each dataset was validated both technically and biologically, and in each case tested we found our RNA-Seq observations to have high predictive validity. We propose this dataset, and the accompanying analysis, to be a helpful resource in the understanding of changes in gene expression during development.
Overall design Three young adult cerebral cortices four embryonic cerebral cortices
Contributor(s) Dillman AA, Hauser DN, Gibbs JR, Nalls MA, McCoy M, Kaganovich A, Rudenko IN, Galter D, Cookson MR
Citation(s) 23416452
Submission date Aug 03, 2012
Last update date May 15, 2019
Contact name J Raphael Gibbs
Organization name National Institutue on Aging, NIH
Department Computational Biology Core
Lab Laboratory of Neurogenetics
Street address 35 Convent Drive, Bldg 35/1A1015, MSC3707
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (7)
GSM980532 Embryonic1
GSM980533 Embryonic2
GSM980534 Embryonic3
BioProject PRJNA171892
SRA SRP014662

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Supplementary file Size Download File type/resource
GSE39866_Mouse.VarianceStabilized.Dataexon.txt.gz 2.1 Mb (ftp)(http) TXT
GSE39866_Mouse.VarianceStabilized.Datagene.txt.gz 937.2 Kb (ftp)(http) TXT
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