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Series GSE39759 Query DataSets for GSE39759
Status Public on May 01, 2013
Title Traumatic Brain Injury Induces Macrophage Subsets In The Brain
Organism Mus musculus
Experiment type Expression profiling by array
Summary We compared arginase-1+ macrophages (macrophages were defined by flow cytometry as CD45hi CD11b+ Ly6G-) with arginase-1- brain macrophages following traumatic brain injury (TBI) by isolating these cells from YARG transgenic mice, which express YFP under the arginase-1 promoter. Both cell populations were isolated from YARG brain tissues one day following TBI. We also examined the expression profile of peripheral blood monocytes (monocytes were defined by flow cytometry as CD11bhi F4/80+) from injured YARG mice and from normal YARG mice. Peripheral blood samples were compared to TBI brain macrophages to assess gene expression changes before and after infiltration into the brain.
TBI macrophage subsets were identified by using a reporter mouse strain (YARG) that expresses eYFP from an IRES inserted at the 3' end of the gene for arginase-1 (Arg1), a hallmark of alternatively activated (M2) macrophages. One day after TBI, 21±1.5% of ipsilateral brain macrophages expressed relatively high levels of Arg1 as detected by YFP. Gene expression analysis of Arg1+ and Arg1- brain macrophage populations revealed that these populations were distinct from either classically activated (M1) macrophages or M2 macrophages, with features of both. The Arg1+ cells differed from Arg1- cells in multiple aspects, most notably in their chemokine repertoires. Thus, the macrophage response to TBI involves recruitment of at least two major macrophage subsets. Overall, our data indicate that the macrophage response to TBI is heterogeneous and unique.
Overall design Four groups (Arg1- brain macrophages post-TBI, Arg1+ brain macrophages post-TBI, normal blood monocytes, blood monocytes post-TBI) were analyzed. Four replicates of each group were analyzed for a total of 16 samples (only 3 replicates of the blood monocyte groups are included in this submission).
Contributor(s) Seaman WE, Hsieh CL, Nakamura MC, Niemi EC
Citation(s) 23630120
Submission date Jul 30, 2012
Last update date Jan 12, 2017
Contact name Christine L. Hsieh
Phone 415 750-2104
Organization name UC San Francisco/ San Francisco VA Medical Center
Department Immunology
Lab William E. Seaman
Street address 4150 Clement St. 111R
City San Francisco
State/province CA
ZIP/Postal code 94121
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (14)
GSM978798 Arg1- brain macrophages_post-TBI day 1_rep 1
GSM978799 Arg1- brain macrophages_post-TBI day 1_rep 2
GSM978800 Arg1- brain macrophages_post-TBI day 1_rep 3
BioProject PRJNA171589

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39759_RAW.tar 30.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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