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Series GSE39666 Query DataSets for GSE39666
Status Public on Jul 27, 2012
Title Tet2 facilitates the de-repression of myeloid target genes during C/EBPa induced transdifferentiation of pre-B cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Tet2 is an enzyme that hydroxylates methylated cytosines and has been implicated in hematopoietic differentiation and the formation of myeloid malignancies when mutated. An ideal system to study the role of Tet2 in myelopoeisis is C/EBPa induced transdifferentiation of pre-B cells into macrophages, where many myeloid genes become rapidly upregulated. Here we found that C/EBPa binds to upstream regions of Tet2 and that the gene becomes activated. Tet2 knockdowns impaired the upregulation of macrophage markers as well as phagocytic capacity, suggesting that the enzyme is required for both early and late stages of myeloid differentiation. A slightly weaker effect was seen in primary cells with a Tet2 ablation. Expression arrays of transdifferentiating cells with Tet2 knockdowns permitted the identification of a small subset of myeloid genes whose upregulation was blunted. Activation of these target genes was accompanied by a rapid increase of promoter hydroxy-methylation. Our observations indicate that Tet2 helps C/EBPa to rapidly de-repress myeloid genes during the conversion of pre-B cells into macrophages.
Overall design Gene expression was measured in a control and Tet2kd mouse preB cell line (called Haftl) harboring an inducible form of C/EBPa. Expression was measured in starting cells and cells induced to transdifferentiate into macrophage-like cells for 24 hours. Technical duplicates for each treatment and time were assayed.
Contributor(s) Kallin EM, Graf T
Citation(s) 22981865
Submission date Jul 26, 2012
Last update date Jan 19, 2018
Contact name Eric M. Kallin
Organization name Center for Genomic Regulation
Department Differentiation and Cancer
Lab Thomas Graf
Street address C/ Dr. Aiguader, 88
City Barcelona
State/province Barcelona
ZIP/Postal code 08003
Country Spain
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (8)
GSM977053 Control_C10_0h_1
GSM977054 Control_C10_0h_2
GSM977055 Control_C10_24h_1
BioProject PRJNA171355

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Supplementary file Size Download File type/resource
GSE39666_RAW.tar 76.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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