|
| Status |
Public on Nov 21, 2012 |
| Title |
Site-specific silencing of regulatory elements as a mechanism of X-inactivation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The inactive X chromosome’s (Xi) physical territory is microscopically devoid of transcriptional hallmarks and enriched in silencing-associated modifications. How these microscopic signatures relate to specific Xi sequence is unknown. This study reports the profiling of Xi gene expression and chromatin states at high-resolution via allele-specific sequencing in F1 hybrid mouse trophoblast stem cells (TSCs). Datasets provided include those generated from strand-specific RNA-Seq, ChIP-Seq, FAIRE-Seq, and DNase-Seq protocols. Included for each dataset are FASTQ files, BED alignments and WIG files with coordinates relative to UCSC genome build mm9, and _snp files that report the location of all SNP-overlapping reads.
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| |
|
| Overall design |
The F1 TSC lines profiled were generated from crosses between CAST/EiJ (Cast) and C57BL/6J (B6) mice. C/B denotes a Cast mother and B6 father, and B/C denotes a B6 mother and Cast father.
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| |
|
| Contributor(s) |
Calabrese JM |
| Citation(s) |
23178118 |
| Submission date |
Jul 16, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
J Mauro Calabrese |
| E-mail(s) |
jmcalabr@med.unc.edu
|
| Phone |
919-843-3257
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Pharmacology
|
| Street address |
120 Mason Farm Road
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27516 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (17)
|
|
| Relations |
| BioProject |
PRJNA170844 |
| SRA |
SRP014437 |
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