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| Status |
Public on Oct 01, 2012 |
| Title |
Cohesin association to replication sites depends on Rad50 and promotes fork restart |
| Platform organism |
Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae W303 |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
Other
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| Summary |
The cohesin complex holds together newly-replicated chromatids and is involved in diverse pathways that preserve genome integrity. We show that in budding yeast, cohesin is transiently recruited to active replication origins and it spreads along DNA as forks progress. When DNA synthesis is impeded, cohesin accumulates at replication sites and is critical for the recovery of stalled forks. Cohesin enrichment at replication forks does not depend on H2A(X) formation, which differs from its loading requirements at DNA double-strand breaks (DSBs). However, cohesin localization is largely reduced in rad50delta mutants and cells lacking both Mec1 and Tel1 checkpoint kinases. Interestingly, cohesin loading at replication sites depends on the structural features of Rad50 that are important for bridging sister chromatids, including the CXXC hook domain and the length of the coiled-coil extensions. Together, these data reveal a novel function for cohesin in the maintenance of genome integrity during S phase.
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| Overall design |
Scc1 ChIP, Rad50 ChIP and BrdU IP in wild type and mutants at different stages of the cell cycle.
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| Contributor(s) |
Tittel-Elmer M, Lengronne A, Davidson MB, Bacal J, François P, Hohl M, Petrini JH, Pasero P, Cobb JA |
| Citation(s) |
22885006 |
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| Submission date |
Jul 03, 2012 |
| Last update date |
Jan 09, 2016 |
| Contact name |
Philippe Pasero |
| E-mail(s) |
ppasero@igh.cnrs.fr
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| Organization name |
Institute of Human Genetics - CNRS
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| Lab |
Pasero
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| Street address |
141 rue de la Cardonille
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| City |
Montpellier |
| ZIP/Postal code |
34396 |
| Country |
France |
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| Platforms (1) |
| GPL7250 |
[Sc03b_MR] Affymetrix GeneChip S. cerevisiae Tiling 1.0R Array |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA170884 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE39078_RAW.tar |
1.4 Gb |
(http)(custom) |
TAR (of BAR, CEL) |
| Processed data provided as supplementary file |
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