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Series GSE38845 Query DataSets for GSE38845
Status Public on Jun 20, 2015
Title Regulatory mechanisms on the early process of mouse spermatogenesis investigated by digital gene expression tag profiling analysis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A detailed understanding of the gene regulation mechanisms in premeiosis cells is germane to understanding normal spermatogenesis. The genome-wide expression profiles of type B spermatogonia and primary spermatocytes in mouse were investigated using Solexa/Illumina’s digital gene expression (DGE) system, a tag based high-throughput transcriptome sequencing method, and the developmental process of early spermatogenesis was analyzed systematically. The results suggested that the expression pattern of mouse type B spermatogonia and primary spermatocytes was changed dramatically, especially the genes related to junction assembly, regulation of actin cytoskeleton and pluripotency. The change of the expression levels of these pathways’ main components may be affected by corresponding miRNAs through post-transcriptional regulation. The analysis results will benefit for better understanding the molecular mechanism of early spermatogenesis process.
Overall design Using DGE technology to examine gene expression pattern's change between mouse type B spermatogonia and primary spermatocytes cell types.
Contributor(s) Zhang X, Hao L, Meng L, Hu S, Jia M
Citation(s) 23554914
Submission date Jun 21, 2012
Last update date May 15, 2019
Contact name lili hao
Organization name BIG
Street address Beichen west road
City Beijing
ZIP/Postal code 100101
Country China
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM950662 DGE of GC-1spg
GSM950663 DGE of GC-2spd
BioProject PRJNA169095
SRA SRP013848

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38845_RAW.tar 1.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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