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Series GSE38838 Query DataSets for GSE38838
Status Public on Jun 19, 2013
Title Cross-presenting CD103+ dendritic cells are protected from influenza virus infection
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary CD8+ cytotoxic T cells are critical for viral clearance from the lungs upon influenza virus infection. The contribution of cross-presentation to the induction of anti-viral cytotoxic T cells remains debated. Here, we used a recombinant influenza virus expressing a NS1-GFP reporter gene to visualize the route of antigen presentation by lung dendritic cells (DC) upon viral infection in vivo. We found that lung CD103+ DC are the only subset to carry intact GFP protein to the draining lymph nodes. Strikingly, lung migratory CD103+ DC are not productively infected by influenza virus and thus induce virus-specific CD8+ T cells through the cross-presentation of antigens from virally infected cells. We also show that CD103+ DC resistance to infection correlates with an increased antiviral state in these cells that is dependent on the expression of IFN receptor alpha. In conclusion, these results establish that efficient cross-priming by migratory lung DC is coupled to the acquisition of an anti-viral status, which is dependent on type I IFN signaling pathway.
Overall design mRNA profiles were generated by deep-sequencing in Illumina HiSeq2000 from alveolar macrophages and CD103+ dendritic cells from lungs of untreated and flu-treated mice
Contributor(s) Merad M, Brown BD, García-Sastre A
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Submission date Jun 20, 2012
Last update date May 15, 2019
Contact name Brian Brown
Organization name Icahn School of Medicine at Mount Sinai
Department Genetics and Genomic Sciences
Street address 1470 Madison Avenue
City New York
State/province NY
ZIP/Postal code 10029
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (4)
GSM950622 CD103+ dendritic cells from untreated mice
GSM950623 CD103+ dendritic cells from flu-treated mice
GSM950624 Macrophages from untreated mice
BioProject PRJNA169091
SRA SRP013843

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Supplementary file Size Download File type/resource
GSE38838_Flu_consolidated.txt.gz 101.9 Kb (ftp)(http) TXT
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