GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE38779 Query DataSets for GSE38779
Status Public on Feb 06, 2013
Title Host cell subversion by Toxoplasma GRA16, an exported dense granule protein that targets the host cell nucleus and alters gene expression
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by array
Summary After invading host cells, Toxoplasma gondii multiplies within a parasitophorous vacuole (PV) that is maintained by parasite proteins secreted from organelles called dense granules. Most dense granule proteins remain within the PV and few are known to access the host cell cytosol. We identify GRA16 as a dense granule protein that is exported through the PV membrane and reaches the host cell nucleus where it positively modulates genes involved in cell cycle progression and the p53 tumor suppressor pathway. GRA16 binds two host enzymes, the deubiquitinase HAUSP and PP2A phosphatase, which exert several functions, including regulation of p53 and the cell cycle. GRA16 alters p53 levels in a HAUSP-dependent manner and induces nuclear translocation of the PP2A holoenzyme. Additionally, certain GRA16-deficient strains exhibit attenuated virulence, indicating the importance of these host alterations in pathogenesis. Therefore, GRA16 represents a potentially emerging subfamily of exported dense granule proteins that modulate host function.
Overall design Mouse bone marrow-derived macrophages (BMDM) or Human foreskin fibroblasts (HFFs) were infected with the following Toxoplasma gondii strains:
- RHku80 WT versus RHku80(deltaGRA16) mutant (in BMDM)
- Pruku80 WT versus Pruku80(deltaGRA16) mutant (in BMDM)
- RHku80 WT versus RHku80(deltaGRA16) mutant (in HFF)
Contributor(s) Bougdour A, Durandau E, Ortet P, Barakat M, Brenier-Pinchart M, Kieffer S, Curt A, Curt R, Bastien O, Coute Y, Pelloux H, Hakimi MA
Citation(s) 23601110
Submission date Jun 18, 2012
Last update date Nov 01, 2017
Contact name Mohamed-ali HAKIMI
Phone (33)476637469
Organization name CNRS
Department UMR5309
Street address Domaine de la Merci, Campus Santé
State/province Grenoble
ZIP/Postal code 38700
Country France
Platforms (2)
GPL10191 NimbleGen Homo sapiens HG18 090828 opt expr HX12 (12x135k)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (6)
GSM945860 GRA16-dependent host transcriptional responses in mouse BMDM infected with T. gondii [RH_GRA16-KO]
GSM946926 GRA16-dependent host transcriptional responses in mouse BMDM infected with T. gondii [Pru_GRA16-KO]
GSM947409 GRA16-dependent host transcriptional responses HFF infected with T. gondii [RH_GRA16-KO]
BioProject PRJNA168971

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38779_RAW.tar 50.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap