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| Status |
Public on Jun 03, 2015 |
| Title |
Gene expression analysis in sensitive and tolerant Citrus rootstocks under chlorotic conditions |
| Platform organism |
Citrus |
| Sample organisms |
Citrus sinensis x Citrus trifoliata; Citrus x paradisi x Citrus trifoliata |
| Experiment type |
Expression profiling by array
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| Summary |
Iron chlorosis is one of the major abiotic stresses affecting fruit trees and other crops in calcareous soils. The most evident symptoms are connected to a reduction in growth and yield and in the interveinal chlorosis of leaves. A custom CombiMatrix 90K microarray was used to identify candidate genes involved in the citrus response to iron deficiency stress, comparing Tarocco Scirè orange [Citrus sinensis (L.) Osbeck] grafted on two different rootstocks, Swingle citrumelo (C. paradisi × Poncirus trifoliata), high sensitive, and Carrizo citrange (C. sinensis × P. trifoliata), tolerant. RNA was extracted from roots of plants grown in two different soils, one volcanic (0% of active lime) used as control, and the other calcareous (10% of active lime).
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| Overall design |
Root tips of two citrus rootstocks, Carrizo Citrange and Swingle Citrumelo, respectively tolerant and sensitive to iron deficiency, each grown in two different soil (volcanic, considered as a control with 0% of active lime, and chlorotic, with 10% of active lime) were analyzed. Three biological replicates were taken for each sample, resulting in a total of twelve observations.
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| Contributor(s) |
Licciardello C, Torrisi B, Tononi P, Allegra M, Sciacca F, Muccilli V, Delledonne M, Intrigliolo F, Recupero GR |
| Citation |
Concetta Licciardello, Biagio Torrisi, Maria Allegra, Fabiola Sciacca, Giancarlo Roccuzzo, Francesco Intrigliolo, Giuseppe Reforgiato Recupero, Paola Tononi, Massimo Delledonne, and Vera Muccilli. A Transcriptomic Analysis of Sensitive and Tolerant Citrus Rootstocks under Natural Iron Deficiency Conditions. J. Amer. Soc. Hort. Sci. November 2013 138:487-498
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| Submission date |
Jun 12, 2012 |
| Last update date |
Sep 03, 2015 |
| Contact name |
Alberto Ferrarini |
| E-mail(s) |
alberto.ferrarini@univr.it
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| Phone |
+39-045-802-7058
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| Organization name |
University of Verona
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| Department |
Scientific and Technological Department
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| Lab |
Plant Functional Genomics Centre
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| Street address |
Strada le Grazie, 15
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| City |
Verona |
| State/province |
Veneto |
| ZIP/Postal code |
37134 |
| Country |
Italy |
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| Platforms (1) |
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| Samples (12)
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| GSM947314 |
roots Swingle citrumelo vulcanic rep1 |
| GSM947315 |
roots Swingle citrumelo vulcanic rep2 |
| GSM947316 |
roots Swingle citrumelo vulcanic rep3 |
| GSM947317 |
roots Swingle citrumelo calcareous rep1 |
| GSM947318 |
roots Swingle citrumelo calcareous rep2 |
| GSM947319 |
roots Swingle citrumelo calcareous rep3 |
| GSM947320 |
roots Carrizo citrange vulcanic rep1 |
| GSM947321 |
roots Carrizo citrange vulcanic rep2 |
| GSM947322 |
roots Carrizo citrange vulcanic rep3 |
| GSM947323 |
roots Carrizo Citrange calcareous rep1 |
| GSM947324 |
roots Carrizo Citrange calcareous rep2 |
| GSM947325 |
roots Carrizo Citrange calcareous rep3 |
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| Relations |
| BioProject |
PRJNA168363 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38667_RAW.tar |
93.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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