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Series GSE38127 Query DataSets for GSE38127
Status Public on Aug 27, 2012
Title Guthrie card methylomics identifies temporally stable epialleles that are present at birth in humans (Bisulfite-Seq)
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary A major concern in common disease epigenomics is distinguishing causal from consequential epigenetic variation. One means of addressing this issue is to identify the temporal origins of epigenetic variants via longitudinal analyses. However, prospective birth-cohort studies are expensive and time-consuming. Here we report DNA methylomics of archived Guthrie cards for the retrospective longitudinal analyses of in utero-derived DNA methylation variation. We first validate two methodologies for generating comprehensive DNA methylomes from Guthrie cards. Then, using an integrated epigenomic/genomic analysis of Guthrie cards and follow-up samplings, we identify inter-individual DNA methylation variation that is present both at birth and three years later. These findings suggest that disease-relevant epigenetic variation could be detected at birth i.e. before overt clinical disease. Guthrie card methylomics offers a potentially powerful and cost-effective strategy for studying the dynamics of inter-individual epigenomic variation in a range of common human diseases.
Overall design Bisulphite converted DNA was sequenced
Contributor(s) Beyan H, Down TA, Ramagopalan SV, Uvebrant K, Nilsson A, Holland ML, Gemma C, Giovannoni G, Boehm BO, Ebers GC, Lernmark A, Cilio CM, Leslie RD, Rakyan VK
Citation(s) 22919074
Submission date May 22, 2012
Last update date May 15, 2019
Contact name Thomas A Down
Organization name University of Cambridge
Department WT/CRUK Gurdon Institute
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QN
Country United Kingdom
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (6)
GSM935138 genomic DNA from sample F1 dried blood spot (Bisulfite-Seq)
GSM935139 genomic DNA from sample F2 dried blood spot (Bisulfite-Seq)
GSM935140 genomic DNA from sample M dried blood spot (Bisulfite-Seq)
This SubSeries is part of SuperSeries:
GSE38128 Guthrie card methylomics identifies temporally stable epialleles that are present at birth in humans
BioProject PRJNA167305
SRA SRP013310

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Supplementary file Size Download File type/resource
GSE38127_RAW.tar 12.6 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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