|
| Status |
Public on Feb 01, 2006 |
| Title |
Rapid alteration of microRNA levels by histone deacetylase inhibition |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Comparison of miRNA expression profiles in SKBr3 breast cancer cells treated with HDAC inhibitor LAQ-824 vs. untreated SKBr3 cells.
Keywords: Expression profiling of pharmacological response to HDAC inhibitor
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| |
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| Overall design |
MicroRNA expression was compared between treated/untreated SKBr3 breast cancer cells to examine the effects of the HDAC inhibitor LAQ-824 on miRNA expression.
SKBr3 control (untreated breast cancer cell line)
SKBr3 treated (breast cancer cell line treated with 20 uM LAQ-824 for 5 hours)
Both cell lines were grown to ~70% confluency and harvested for enriched miRNAs using the mirVana miRNA Isolation kit from Ambion per the manufacturers instructions. Untreated SKBr3 cell miRNA was labeled with Cy3 and treated SKBr3 treated cell miRNA was labeled with Cy5.
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| |
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| Contributor(s) |
Scott GK, Mattie MD, Berger CE, Benz SC, Benz CC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 09, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Michael D. Mattie |
| E-mail(s) |
mdmattie@yahoo.com
|
| Phone |
919-483-5617
|
| Fax |
919-315-0158
|
| Organization name |
GlaxoSmithKline
|
| Department |
Genomics Research
|
| Lab |
Quantitative Expression
|
| Street address |
Five Moore Drive
|
| City |
Research Triangle Park |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (4)
|
| GSM87296 |
Controlvs.treated_LAQ-824_5hrs_rep1 |
| GSM87297 |
Controlvs.treated_LAQ-824_5hrs_rep2 |
| GSM87298 |
Controlvs.treated_LAQ-824_5hrs_rep3 |
| GSM87299 |
Controlvs.treated_LAQ-824_5hrs_rep4 |
|
| Relations |
| BioProject |
PRJNA93999 |
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