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Series GSE37966 Query DataSets for GSE37966
Status Public on Aug 27, 2012
Title Guthrie card methylomics identifies temporally stable epialleles that are present at birth in humans (Methylation BeadChip)
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary A major concern in common disease epigenomics is distinguishing causal from consequential epigenetic variation. One means of addressing this issue is to identify the temporal origins of epigenetic variants via longitudinal analyses. However, prospective birth-cohort studies are expensive and time-consuming. Here we report DNA methylomics of archived Guthrie cards for the retrospective longitudinal analyses of in utero-derived DNA methylation variation. We first validate two methodologies for generating comprehensive DNA methylomes from Guthrie cards. Then, using an integrated epigenomic/genomic analysis of Guthrie cards and follow-up samplings, we identify inter-individual DNA methylation variation that is present both at birth and three years later. These findings suggest that disease-relevant epigenetic variation could be detected at birth i.e. before overt clinical disease. Guthrie card methylomics offers a potentially powerful and cost-effective strategy for studying the dynamics of inter-individual epigenomic variation in a range of common human diseases.
Overall design Bisulphite converted DNA was hybridised to the Illumina Infinium 450k Human Methylation Beadchip
Contributor(s) Beyan H, Down TA, Ramagopalan SV, Uvebrant K, Nilsson A, Holland ML, Gemma C, Giovannoni G, Boehm BO, Ebers GC, Lernmark A, Cilio CM, Leslie RD, Rakyan VK
Citation(s) 22919074
Submission date May 14, 2012
Last update date Mar 22, 2019
Contact name Thomas A Down
Organization name University of Cambridge
Department WT/CRUK Gurdon Institute
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QN
Country United Kingdom
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (7)
GSM931109 genomic DNA from sample F1 dried blood spot (BeadChip)
GSM931110 genomic DNA from sample F2 dried blood spot (BeadChip)
GSM931111 genomic DNA from sample M dried blood spot (BeadChip)
This SubSeries is part of SuperSeries:
GSE38128 Guthrie card methylomics identifies temporally stable epialleles that are present at birth in humans
BioProject PRJNA167304

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37966_RAW.tar 183.1 Mb (http)(custom) TAR
GSE37966_matrix-non-normalized.txt.gz 16.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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