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Series GSE37930 Query DataSets for GSE37930
Status Public on Dec 31, 2012
Title Fbxl10 regulates PRC1 recruitment to CpG islands and H2A ubiquitination
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Polycomb repressive complex 1 (PRC1) catalyzes H2A monoubiquitination (uH2A) and regulates pluripotency in embryonic stem cells (ESCs). However the mechanisms controlling PRC1 recruitment and activity are largely unknown. Here we show that Fbxl10 interacts with Ring1B and Nspc1, forming a non-canonical PRC1. We demonstrate that Fbxl10-PRC1 is essential for H2A ubiquitination in mouse ESCs. Genome-wide analyses reveal that Fbxl10 preferentially binds to CpG islands and co-localizes with Ring1B on Polycomb target genes. Notably, Fbxl10 depletion causes modest dissociation of Ring1B but a major loss of uH2A on target genes. Furthermore rescue experiments for Fbxl10 reveal that its DNA binding capability and integration into PRC1 are required for proper H2A ubiquitination. ES cells lacking Fbxl10, like previously characterized Polycomb mutants, show a severely compromised capacity for successful differentiation. Our results shed light on a novel mechanism how CpG islands regulate chromatin function by affecting polycomb recruitment and activity.
 
Overall design All ChIP-seq reactions were performed in either untransfected cells, cells expressing scrambled shRNA or Fbxl10 shRNA, Ring1b-/- or Suz12-/- mouse ES cells
 
Contributor(s) Wu X, Johansen JV
Citation(s) 23395003
Submission date May 11, 2012
Last update date May 15, 2019
Contact name Xudong Wu
E-mail(s) xudong.wu@bric.ku.dk
Organization name Biotech Research and Innovation Centre
Street address Ole Maal√łes Vej 5
City Copenhagen
ZIP/Postal code 2200
Country Denmark
 
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (11)
GSM930141 Fbxl10_Scr
GSM930142 Fbxl10_shFbxl10
GSM930143 Ring1B_Scr_exp1
Relations
SRA SRP013052
BioProject PRJNA166917

Download family Format
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Supplementary file Size Download File type/resource
GSE37930_RAW.tar 3.3 Gb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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