|
| Status |
Public on Sep 05, 2012 |
| Title |
Murine CD8+ T cells: in vitro-derived vs. thymocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling of mouse in-vitro derived CD8+ cells (obtained after culturing BM-HSCs isolated from B6 mice) with OP9-DL1 cells for 35 days were compared to CD8+ thymocytes isolated from 4-5 weeks old B6 mice. Our goal was to determine similarities and differences in gene expression profile between in vitro-derived CD8+ cells and CD8+ cells isolated from the thymus. The procedure of in vitro differentiation of HSC using OP9-DL1 cocultures was previously described by: Schmitt, T. M., and J. C. Zuniga-Pflucker. 2002. Induction of T cell development from hematopoietic progenitor cells by delta-like-1 in vitro. Immunity 17:749-756 and Holmes, R., and J. C. Zuniga-Pflucker. 2009. The OP9-DL1 system: generation of T-lymphocytes from embryonic or hematopoietic stem cells in vitro. Cold Spring Harb Protoc 2009:pdb prot5156.
|
| |
|
| Overall design |
Biological replicates: 2 different samples of in vitro-derived CD8+ T cells from day 35 were compared to 2 different samples of thymic CD8+ cells.
|
| |
|
| Contributor(s) |
Dervovic D, Zuniga-Pflucker JC, Kianizad K |
| Citation(s) |
22925927 |
| |
| Submission date |
Apr 30, 2012 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Dzana Dzenetdina Dervovic |
| E-mail(s) |
d.dervovic@utoronto.ca
|
| Phone |
1 416 480-6100 7340
|
| Fax |
1 416 480-4375
|
| Organization name |
Sunnybrook Research Institute
|
| Department |
Molecular and Cellular Biology
|
| Lab |
Immunology
|
| Street address |
2075 Bayview Av.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M4N 3M5 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
|
| Samples (4)
|
| GSM925275 |
thymic CD8+ T-cell prep1_vs_OP9 CD8+ T-cell prep1 |
| GSM925276 |
thymic CD8+ T-cell prep1_vs_OP9 CD8+ T-cell prep2 |
| GSM925277 |
thymic CD8+ T-cell prep2_vs_OP9 CD8+ T-cell prep1 |
| GSM925278 |
thymic CD8+ T-cell prep2_vs_OP9 CD8+ T-cell prep2 |
|
| Relations |
| BioProject |
PRJNA162585 |
Less...
More...