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Series GSE37669 Query DataSets for GSE37669
Status Public on Sep 05, 2012
Title Murine CD8+ T cells: in vitro-derived vs. thymocytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary Transcriptional profiling of mouse in-vitro derived CD8+ cells (obtained after culturing BM-HSCs isolated from B6 mice) with OP9-DL1 cells for 35 days were compared to CD8+ thymocytes isolated from 4-5 weeks old B6 mice. Our goal was to determine similarities and differences in gene expression profile between in vitro-derived CD8+ cells and CD8+ cells isolated from the thymus. The procedure of in vitro differentiation of HSC using OP9-DL1 cocultures was previously described by: Schmitt, T. M., and J. C. Zuniga-Pflucker. 2002. Induction of T cell development from hematopoietic progenitor cells by delta-like-1 in vitro. Immunity 17:749-756 and Holmes, R., and J. C. Zuniga-Pflucker. 2009. The OP9-DL1 system: generation of T-lymphocytes from embryonic or hematopoietic stem cells in vitro. Cold Spring Harb Protoc 2009:pdb prot5156.
Overall design Biological replicates: 2 different samples of in vitro-derived CD8+ T cells from day 35 were compared to 2 different samples of thymic CD8+ cells.
Contributor(s) Dervovic D, Zuniga-Pflucker JC, Kianizad K
Citation(s) 22925927
Submission date Apr 30, 2012
Last update date Jan 12, 2017
Contact name Dzana Dzenetdina Dervovic
Phone 1 416 480-6100 7340
Fax 1 416 480-4375
Organization name Sunnybrook Research Institute
Department Molecular and Cellular Biology
Lab Immunology
Street address 2075 Bayview Av.
City Toronto
State/province Ontario
ZIP/Postal code M4N 3M5
Country Canada
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (4)
GSM925275 thymic CD8+ T-cell prep1_vs_OP9 CD8+ T-cell prep1
GSM925276 thymic CD8+ T-cell prep1_vs_OP9 CD8+ T-cell prep2
GSM925277 thymic CD8+ T-cell prep2_vs_OP9 CD8+ T-cell prep1
BioProject PRJNA162585

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37669_RAW.tar 57.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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