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Series GSE37575 Query DataSets for GSE37575
Status Public on Jul 12, 2012
Title Tumor-derived Tissue Factor Activates Coagulation and Enhances Thrombosis in a Mouse Xenograft Model of Human Pancreatic Cancer
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cancer patients often have an activated clotting system and are at increased risk for venous thrombosis. In this study, we analyzed tissue factor (TF) expression in four different human pancreatic tumor cell lines for the purpose of producing derivative tumors in vivo. We found that two of the lines expressed TF and released TF-positive microparticles (MPs) into the culture medium. The majority of TF protein in the culture medium was associated with MPs. Importantly, only TF-positive cell lines activated coagulation in nude mice, and this activation was abolished by an anti-human TF antibody. Of the two TF-positive lines only one produced detectable levels of human MP TF activity in the plasma when grown orthotopically in nude mice. Surprisingly, <5% of human TF protein in plasma from tumor-bearing mice was associated with MPs. Mice with TF-positive tumors and elevated levels of circulating TF-positive MPs had increased thrombosis in a saphenous vein model. In contrast, we observed no difference in thrombus weight between tumor bearing and control mice in an inferior vena cava stenosis model. Our studies suggest that in a xenograft mouse model tumor TF activates coagulation, whereas TF on circulating MPs may trigger venous thrombosis.
Overall design 8 human tumor pancreatic cell lines
Contributor(s) Wang J, Yeh J, Mackman N
Citation(s) 22547577
Submission date Apr 25, 2012
Last update date Feb 22, 2018
Contact name Jen Jen Yeh
Organization name University of North Carolina at Chapel Hill
Department Surgical Oncology
Lab Yeh Lab
Street address LCCC, 450 West Drive
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (12)
GSM922134 Capan2
GSM922135 HCT-116
GSM922136 HPAC
BioProject PRJNA162557

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37575_RAW.tar 6.9 Mb (http)(custom) TAR
Processed data included within Sample table

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