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Series GSE37525 Query DataSets for GSE37525
Status Public on Dec 15, 2012
Title Genome-wide identification of enhancers in skeletal muscle
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In an effort to identify the compendium of regulatory elements that govern myogenic differentiation, we generated chromatin state maps based on the recruitment of factors (p300 and PolII) and histone modifications (H3K4me1, H3K27ac) that typify enhancers in myoblasts and myotubes. We found a remarkable concordance between the location of these newly defined, active enhancers and MyoD1 binding events. In addition, we found a striking association between the position of enhancers and RNA polymerase II recruitment, which resulted in the expression of previously disclosed non-coding RNAs, as well as potentially new transcripts identified ab initio, throughout the enhancer region.
Overall design Mapping of H3K27ac and p300 in growing myoblasts (MB) and fully differentiated myotubes (MT). Mapping of c-Jun in growing myoblasts (MB).
Contributor(s) Blum R, Vethantham V, Bowman C, Rudnicki M, Dynlacht BD
Citation(s) 23249738
Submission date Apr 23, 2012
Last update date May 15, 2019
Contact name Brian Dynlacht
Organization name NYU Langone Medical Center
Street address 550 1st avenue
City New York
ZIP/Postal code 10016
Country USA
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (14)
GSM921130 H3K27ac_MB_replicate1
GSM921131 H3K27ac_MB_replicate2
GSM921132 H3K27ac_MT_replicate1
BioProject PRJNA161011
SRA SRP012465

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37525_MB_H3K27ac_peaks.bed.gz 1.6 Mb (ftp)(http) BED
GSE37525_MB_c-Jun_peaks.bed.gz 155.8 Kb (ftp)(http) BED
GSE37525_MB_p300_peaks.bed.gz 942.1 Kb (ftp)(http) BED
GSE37525_MT_H3K27ac_peaks.bed.gz 2.0 Mb (ftp)(http) BED
GSE37525_MT_p300_peaks.bed.gz 861.8 Kb (ftp)(http) BED
GSE37525_RAW.tar 1.0 Gb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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