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Series GSE37430 Query DataSets for GSE37430
Status Public on Mar 01, 2013
Title Gene regulation following MIF / IL-8 stimulation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Chronic lymphocytic leukemia (CLL) is characterized by the accumulation of CD5+ B lymphocytes in peripheral blood, lymphoid organs and BM. The main feature of the disease is accumulation of the malignant cells due to decreased apoptosis. CD84 belongs to the Signaling Lymphocyte Activating Molecule (SLAM) family of immunoreceptors, and has an unknown function in CLL cells. Here, we show that the expression of CD84 is significantly elevated from the early stages of the disease, and is regulated by macrophage migration inhibitory factor (MIF) and its receptor, CD74. Activation of cell surface CD84 initiates a signaling cascade that enhances CLL cell survival. Both immune-mediated neutralization or blockade of CD84 induce cell death in vitro and in vivo. In addition, analysis of samples derived from an on-going clinical trial, in which human subjects were treated with humanized anti-CD74 milatuzumab shows a decrease in CD84 mRNA levels milatuzumab-treated cells. This downregulation was correlated with reduction of Bcl-2 and Mcl-1 message. Thus, our data show that overexpression of CD84 in CLL is an important survival mechanism that appears to be an early event in the pathogenesis of the disease. These findings suggest novel therapeutic strategies based on the blockade of this CD84-dependent survival pathway.
Overall design 3 samples; incubated with PBS, MIF, or IL-8.
Contributor(s) Inbal B
Citation(s) 23435417
Submission date Apr 19, 2012
Last update date Dec 06, 2018
Contact name Idit Shachar
Organization name Weizmann Institute of Science
Street address 2 Hersel st
City Rehovot
ZIP/Postal code 76100
Country Israel
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (3)
GSM919191 Cells incubated with PBS
GSM919192 Cells incubated with MIF
GSM919193 Cells incubated with IL-8
BioProject PRJNA159911

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37430_IS25-IS24.txt.gz 24.8 Kb (ftp)(http) TXT
GSE37430_IS26-IS24.txt.gz 25.6 Kb (ftp)(http) TXT
GSE37430_IS26-IS25.txt.gz 9.5 Kb (ftp)(http) TXT
GSE37430_RAW.tar 6.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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