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Series GSE37361 Query DataSets for GSE37361
Status Public on Jan 01, 2013
Title Impact of Ino-C2-PAF on the gene expression profile of HaCaT cells after stimulation with pro-inflammatory cytokines
Organism Homo sapiens
Experiment type Expression profiling by array
Summary New alkyl-phospholipids (APLs) that are structurally derived from the platelet-activating factor are promising candidates for anticancer treatment. After the incorporation into cell membranes, APLs are able to interfere with a wide variety of key enzymes implicated in cell growth, motility, invasion and apoptosis. Recently, using Agilent cDNA microarray technology, we could demonstrate that the glycosidated APL Ino-C2-PAF inhibited the expression of several genes associated with immune response and inflammation in immortalized keratinocytes HaCaT. Here, we analyzed the impact of Ino-C2-PAF on the gene expression profile of HaCaT cells treated with several pro-inflammatory cytokines (IL-1α, IL-17, IL-22, TNF-α and oncostatin-M).
Overall design The influence of Ino-C2-PAF on the transcriptional profile of immortalized keratinocytes (HaCaT) was analyzed treating HaCaT cells with 5 µM Ino-C2-PAF in the presence of a mixture of pro-inflammatory cytokines (IL-1α, IL-17, IL-22, TNF-α and oncostatin-M) for 24 h. Control cells were left untreated. Three independent experiments were performed for each condition, except for control cells where only two experiments were performed. Control cells were mainly necessary to demonstrate the efficiency of this in vitro inflammation model for keratinocytes.
Contributor(s) Semini G, Klein A, Lucka L, Danker K
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Submission date Apr 17, 2012
Last update date Nov 27, 2018
Contact name Geo Semini
Organization name Charité - Universitätmedizin
Department Institute for Biochemie
Street address Oudenarderstrasse 16
City Berlin
ZIP/Postal code 13347
Country Germany
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (8)
GSM916679 HaCaT_Control_Rep1
GSM916680 HaCaT_Control_Rep2
GSM916681 HaCaT_Cytokines_Rep1
BioProject PRJNA159561

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37361_RAW.tar 102.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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