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Series GSE37350 Query DataSets for GSE37350
Status Public on Apr 18, 2012
Title Altered Subcellular Localization of Transcription Factor TEAD4 Regulates First Mammalian Cell Lineage Commitment
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In the preimplantation mouse embryo TEAD4 is critical to establishing the trophectoderm (TE)-specific transcriptional program and segregating TE from the inner cell mass (ICM). However, TEAD4 is expressed both in the TE and the ICM. Thus, differential function of TEAD4 rather than expression itself regulates specification of the first two cell lineages. We used ChIP-seq to define genome-wide TEAD4 target genes and asked how transcription of TEAD4 target genes is specifically maintained in the TE. Our analyses revealed an evolutionarily conserved mechanism, in which lack of nuclear localization of TEAD4 impairs the TE-specific transcriptional program in inner blastomeres, thereby allowing their maturation towards the ICM lineage. Restoration of TEAD4 nuclear localization maintains the TE-specific transcriptional program in the inner blastomeres and prevents segregation of the TE and ICM lineages and blastocyst formation. We propose that altered subcellular localization of TEAD4 in blastomeres dictates first mammalian cell fate specification.
Overall design ChIPseq profiles of TEAD4, IgG, Input in Mouse trophoblast stem cells using Illumina HiSeq 2000 and Illumina Genome Analyzer IIx
Contributor(s) Home P, Gunewardena S, Yoo B, Schulz V, Gallagher PG, Paul S
Citation(s) 22529382
Submission date Apr 17, 2012
Last update date May 15, 2019
Contact name Byunggil Yoo
Organization name KUMC
Street address 3901 Rainbow Blvd
City Kansas City
State/province KS
ZIP/Postal code 66202
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (3)
GSM916551 TEAD4
GSM916552 IgG
GSM916553 Input DNA
SRA SRP012267
BioProject PRJNA159725

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Supplementary file Size Download File type/resource
GSE37350_RAW.tar 851.8 Mb (http)(custom) TAR (of BW) 125.8 Kb (ftp)(http) BB
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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