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Series GSE37235 Query DataSets for GSE37235
Status Public on Jun 07, 2012
Title The Stat3/GR interaction code: predictive value of direct/indirect DNA recruitment for transcription outcome
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Transcription factor recruitment to genomic sites of action is primarily due to direct protein:DNA interactions. The subsequent recruitment of co-regulatory complexes leads to either transcriptional activation or repression. In contrast to this canonical scheme, some transcription factors such as the glucocorticoid receptor (GR) behave as transcriptional repressors when recruited to target genes through protein tethering. We have investigated the genome-wide prevalence of tethering between GR and Stat3 and found non-reciprocal interactions, namely that GR tethering to DNA-bound Stat3 results in transcriptional repression whereas Stat3 tethering to GR results in synergism. Further, other schemes of GR and Stat3 co-recruitment to regulatory modules result in transcriptional synergism, including neighbouring and composite binding sites. The results indicate extensive transcriptional interactions between Stat3 and GR; further, they provide a genome-wide assessment of transcriptional regulation by tethering and a molecular basis for integration of signals mediated by GR and Stats in health and disease.
Overall design ChIP-seq analysis of Stat3 and GR binding sites in pituitary corticotroph AtT-20 cell model. Five independant ChIPs were pooled prior library preparation for each contitions: STAT3 (LIF), STAT3 (Dex+LIF), GR (Dex+LIF), GR (Dex) and control IgG.
Contributor(s) Langlais D, Couture C, Drouin J
Citation(s) 22633955
Submission date Apr 12, 2012
Last update date May 15, 2019
Contact name David Langlais
Organization name McGill University
Department Human Genetics
Lab Inflammation Genomics Lab
Street address 740 Ave Dr Penfield, rm 4203, McGill Genome Centre
State/province QC
ZIP/Postal code H3A0G1
Country Canada
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (5)
GSM926623 Control IgG
GSM926624 Stat3 (LIF)
GSM926625 Stat3 (Dex+LIF)
BioProject PRJNA163115
SRA SRP012641

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Supplementary file Size Download File type/resource
GSE37235_RAW.tar 2.3 Gb (http)(custom) TAR (of WIG)
GSE37235_S3-GR_peak_cluster_list.bed.gz 62.7 Kb (ftp)(http) BED
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Processed data provided as supplementary file
Raw data are available in SRA
Processed data are available on Series record

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