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Series GSE37066 Query DataSets for GSE37066
Status Public on Sep 24, 2012
Title Pluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes [Illumina]
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Pluripotent stem cells evade replicative senescence, whereas other primary cells lose their proliferation and differentiation potential after a limited number of cell divisions – and this is accompanied by specific senescence-associated DNA methylation (SA-DNAm) changes. Here, we investigate SA-DNAm changes in mesenchymal stromal cells (MSC) upon long-term culture, irradiation-induced senescence, immortalization and reprogramming into induced pluripotent stem cells (iPSC) using high density HumanMethylation450 BeadChips. SA-DNAm changes are highly reproducible and occur particularly in intergenic and non-promoter regions of developmental genes. We demonstrate that ionizing irradiation, although associated with a very similar senescence phenotype, does not affect SA-DNAm. Furthermore, overexpression of the catalytic subunit of the human telomerase (TERT) or conditional immortalization with a doxycycline-inducible system (TERT and SV40 TAg) result in telomere extension but do not influence SA-DNAm. In contrast, we demonstrate that reprogramming into iPSC prevented SA-DNAm changes. Our results indicate that replicative senescence is associated with an epigenetically controlled process which stalls cells in a particular differentiated state, whereas irradiation-induced senescence and immortalization are not causally related to this process. Absence of SA-DNAm in pluripotent cells may play a central role for their escape from cellular senescence.
Overall design Samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip
Contributor(s) Koch CM, Reck K, Shao K, Lin Q, Ziegler P, Joussen S, Walenda G, Drescher W, Opalka B, May T, Brümmendorf T, Zenke M, Saric T, Wagner W
Citation(s) 23032973, 23080539
Submission date Apr 05, 2012
Last update date Mar 22, 2019
Contact name Wolfgang Wagner
Phone +49 241 8088611
Organization name RWTH Aachen University
Department Helmholtz Institute for Biomedical Engineering
Lab Stem Cell Biology and Cellular Engineering
Street address Pauwelsstrasse 20
City Aachen
ZIP/Postal code 52074
Country Germany
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (24)
GSM853409 MSC donor2
GSM853410 MSC donor4
GSM853411 MSC donor5
This SubSeries is part of SuperSeries:
GSE37067 Pluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes
BioProject PRJNA158007

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37066_RAW.tar 183.1 Mb (http)(custom) TAR
GSE37066_raw_AB_intensities.txt.gz 42.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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