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Series GSE36978 Query DataSets for GSE36978
Status Public on Jul 13, 2012
Title In vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Dicer is an RNase III-family endoribonuclease and haploinsufficient tumor suppressor that is required for the biogenesis of miRNAs, yet in vivo structure-function characterization of its RNase IIIA and IIIB domains have not been reported. In murine Dicer knockout fibroblasts, we expressed human Dicer with point mutations in the RNase III, helicase, and PAZ domains and characterized miRNA expression by Northern blot and massively parallel sequencing of small RNAs. Inactivation of the RNase IIIA or IIIB domain blocked maturation of miRNAs derived from the 3’ or 5’ arms of miRNA precursors, respectively, and resulted in altered miRNA expression profiles.
Overall design Small RNAs from murine mesenchymal stem cells (MScs) with and without Dicer (WT:Dicer f/f, KO:Dicer -/-, KO transfected with various hsDicer point mutants) were analyzed.
Contributor(s) Gurtan AM, Lu V, Bhutkar A, Sharp PA
Citation(s) 22546613
Submission date Apr 02, 2012
Last update date May 15, 2019
Contact name A Bhutkar
Organization name MIT
Street address 77 Massachusetts Avenue
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (16)
GSM907767 WT R1
GSM907768 KO R1
GSM907769 KO Dicer R1
BioProject PRJNA157431
SRA SRP012038

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Supplementary file Size Download File type/resource
GSE36978_RAW.tar 76.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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