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Series GSE36854 Query DataSets for GSE36854
Status Public on Apr 15, 2012
Title Comparison of host cell gene expression in cowpox, monkeypox or vaccinia virus infected cells reveals virus-specific regulation of immune response genes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Orthopoxviruses are large DNA viruses which can cause disease in numerous host species. Even though the eradication of variola virus - the causative agent of human smallpox – succeeded, with the end of vaccinations several other orthopoxviruses emerged as potential threat to human health. For instance, animal-borne monkeypox virus, cowpox virus and closely related vaccinia virus are all capable of establishing zoonotic infections in humans. The disease caused by each virus differs in terms of expression and severity, but we still know little about the reasons for these different phenotypes. They may be explained by the unique repertoire of host cell modulating factors encoded by each virus. In this study, we aimed at characterizing the specific modulation of the host cells gene expression profile by orthopoxvirus infection. In our study we analyzed changes in host cell gene expression of HeLa cells after infection with cowpox virus, monkeypox virus or vaccinia virus and compared these to each other and to the gene expression profile of non-infected cells using Agilent Whole Genome Microarray technology. We could identify major differences in viral modulation of host cell immune response genes, especially an induction of genes involved in leukocyte migration and Toll-like receptor signalling in cowpox and monkeypox virus infected cells. This was not observed following vaccinia virus infection. If these differences contribute to the different clinical manifestation of cowpox, monkeypox and vaccinia virus infections in certain host species remains to be elucidated.
 
Overall design We analyzed the gene expression profile of HeLa cells wich were either mock-infected or infected with Vaccinia virus strain IHD-W, Cowpox virus strain Brighton Red or Monkeypox virus strain MSF#6 at a multiplicity of infection of 5. Experiments were performed in duplicate. At 6 h post infection total RNA was isolated from infected cells and used for microarray analysis.
 
Contributor(s) Bourquain DR, Dabrowski PW, Nitsche A
Citation(s) 23425254
Submission date Mar 27, 2012
Last update date Feb 22, 2018
Contact name Daniel Bourquain
E-mail(s) bourquaind@rki.de
Phone 0049 (0)30187543825
Organization name Robert Koch Institute
Department Centre for Biological Threats and Special Pathogens 1
Lab Highly Pathogenic Viruses
Street address Seestraße 10
City Berlin
State/province Berlin
ZIP/Postal code 13353
Country Germany
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (8)
GSM903055 NK
GSM903056 CPXV
GSM903057 IHD-W
Relations
BioProject PRJNA156905

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36854_RAW.tar 79.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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